Cargando…

Biochemical characterization of the two novel mgCas12a proteins from the human gut metagenome

CRISPR/Cas9 and Cas12a belonging to the Class II CRISPR system are characterized by a single-component effector protein. Despite unique features of Cas12a like DNA cleavage with 5′ staggered ends and a single crRNA, Cas12a has not been adopted in biotechnological applications to the similar extent a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kim, Han Seong, Kim, Dong-wook, Kim, Sungjin, Choe, Sunghwa
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718762/ https://www.ncbi.nlm.nih.gov/pubmed/36460704 http://dx.doi.org/10.1038/s41598-022-25227-w

Ejemplares similares

MG-MLST: Characterizing the Microbiome at the Strain Level in Metagenomic Data
por: Bangayan, Nathanael J., et al.
Publicado: (2020)

Spatial metagenomic characterization of microbial biogeography in the gut
por: Sheth, Ravi U., et al.
Publicado: (2019)

Characterization of the Gut Microbiome Using 16S or Shotgun Metagenomics
por: Jovel, Juan, et al.
Publicado: (2016)

The MG-RAST metagenomics database and portal in 2015
por: Wilke, Andreas, et al.
Publicado: (2016)

Predicting drug-metagenome interactions: Variation in the microbial β-glucuronidase level in the human gut metagenomes
por: Elmassry, Moamen M., et al.
Publicado: (2021)

Expanding the catalog of cas genes with metagenomes
por: Zhang, Quan, et al.
Publicado: (2014)

Metagenomic Analysis of the Gut Microbiota of Wild Mice, a Newly Identified Reservoir of Campylobacter
por: Song, Hyokeun, et al.
Publicado: (2021)

Biochemical and structural characterization of a novel halotolerant cellulase from soil metagenome
por: Garg, Roma, et al.
Publicado: (2016)

Characterization of the gut microbiota of Kawasaki disease patients by metagenomic analysis
por: Kinumaki, Akiko, et al.
Publicado: (2015)

Deep metagenomic characterization of gut microbial community and function in preeclampsia
por: Lv, Li-Juan, et al.
Publicado: (2022)

Identification of brassinosteroid genes in Brachypodium distachyon
por: Corvalán, Claudia, et al.
Publicado: (2017)

Comprehensive 16S rRNA and metagenomic data from the gut microbiome of aging and rejuvenation mouse models
por: Shin, Jongoh, et al.
Publicado: (2022)

Isolation and Biochemical Characterization of a Glucose Dehydrogenase from a Hay Infusion Metagenome
por: Basner, Alexander, et al.
Publicado: (2014)

Molecular and biochemical characterization of a novel isoprene synthase from Metrosideros polymorpha
por: Yeom, Soo-Jin, et al.
Publicado: (2018)

Characterization of a thermostable Cas12a ortholog
por: Wu, Jing, et al.
Publicado: (2023)

Transient expression of hemagglutinin antigen from canine influenza virus H3N2 in Nicotiana benthamiana and Lactuca sativa
por: Maharjan, Puna Maya, et al.
Publicado: (2019)

Plant-Based COVID-19 Vaccines: Current Status, Design, and Development Strategies of Candidate Vaccines
por: Maharjan, Puna Maya, et al.
Publicado: (2021)

Metagenomic-based characterization of the gut virome in patients with polycystic ovary syndrome
por: Huang, Liansha, et al.
Publicado: (2022)

Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
por: Park, Jongjin, et al.
Publicado: (2021)

Metagenomic Surveys of Gut Microbiota
por: Mandal, Rahul Shubhra, et al.
Publicado: (2015)

Metagenomic discovery of novel CRISPR-Cas13 systems
por: Hu, Yanping, et al.
Publicado: (2022)

Molecular characterization of gut microbiome in weaning pigs supplemented with multi-strain probiotics using metagenomic, culturomic, and metabolomic approaches
por: Lee, Woong Ji, et al.
Publicado: (2022)

Interfering with retrotransposition by two types of CRISPR effectors: Cas12a and Cas13a
por: Zhang, Niubing, et al.
Publicado: (2020)

Characterization of Cme and Yme thermostable Cas12a orthologs
por: Fuchs, Ryan T., et al.
Publicado: (2022)

Author Correction: Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins
por: Park, Jongjin, et al.
Publicado: (2021)

A multicenter, randomized, and double-blind phase IV clinical trial to compare the efficacy and safety of fixed-dose combinations of amlodipine orotate/valsartan 5/160 mg versus valsartan/hydrochlorothiazide 160/12.5 mg in patients with essential hypertension uncontrolled by valsartan 160 mg monotherapy
por: Ahn, Youngkeun, et al.
Publicado: (2018)

Soil Metagenome Sequences from a Geothermal Site at Mount Melbourne in Antarctica
por: Park, Mira, et al.
Publicado: (2021)

Comparative pharmacokinetics between two tablets of tramadol 37.5 mg/acetaminophen 325 mg and one tablet of tramadol 75 mg/acetaminophen 650 mg for extended-release fixed-dose combination
por: Kim, Hyun Chul, et al.
Publicado: (2022)

Valsartan 160 mg/Amlodipine 5 mg Combination Therapy versus Amlodipine 10 mg in Hypertensive Patients with Inadequate Response to Amlodipine 5 mg Monotherapy
por: Sung, Jidong, et al.
Publicado: (2016)

Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome
por: De Santi, Concetta, et al.
Publicado: (2016)

Clinical, Biochemical, and Genetic Characterization of Glycogen Storage Type IX in a Child with Asymptomatic Hepatomegaly
por: Kim, Jung Ah, et al.
Publicado: (2015)

Antagonistic Regulation of Arabidopsis Growth by Brassinosteroids and Abiotic Stresses
por: Chung, Yuhee, et al.
Publicado: (2014)

High-Resolution Metagenomics of Human Gut Microbiota Generated by Nanopore and Illumina Hybrid Metagenome Assembly
por: Ye, Lianwei, et al.
Publicado: (2022)

Metagenome of the gut of a malnourished child
por: Gupta, Sourav Sen, et al.
Publicado: (2011)

Metagenomic characterization of the effect of feed additives on the gut microbiome and antibiotic resistome of feedlot cattle
por: Thomas, Milton, et al.
Publicado: (2017)

Characterization of Metagenome-Assembled Genomes and Carbohydrate-Degrading Genes in the Gut Microbiota of Tibetan Pig
por: Zhou, Saisai, et al.
Publicado: (2020)

Characterization of gut microbial and metabolite alterations in faeces of Goto Kakizaki rats using metagenomic and untargeted metabolomic approach
por: Zhao, Jin-Dong, et al.
Publicado: (2023)

The PurR regulon in Escherichia coli K-12 MG1655
por: Cho, Byung-Kwan, et al.
Publicado: (2011)

Biochemical Characterization of the Rice Cinnamyl Alcohol Dehydrogenase Gene Family
por: Park, Hye Lin, et al.
Publicado: (2018)

Darkness and gulliver2/phyB mutation decrease the abundance of phosphorylated BZR1 to activate brassinosteroid signaling in Arabidopsis
por: Kim, Bokyung, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_f7jogoe8b2mrj1qcr6he3dulhg