The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic

SH-SY5Y is a cell line derived from human neuroblastoma. It is one of the most widely used in vitro models to study Parkinson’s disease. Surprisingly, it has been found that it does not develop a dopaminergic phenotype after differentiation, questioning its usefulness as a Parkinson’s model. There a...

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Autores principales: Carvajal-Oliveros, Angel, Uriostegui-Arcos, Maritere, Zurita, Mario, Melchy-Perez, Erika I., Narváez-Padilla, Verónica, Reynaud, Enrique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718961/
https://www.ncbi.nlm.nih.gov/pubmed/36471713
http://dx.doi.org/10.1016/j.ibneur.2022.11.007
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author Carvajal-Oliveros, Angel
Uriostegui-Arcos, Maritere
Zurita, Mario
Melchy-Perez, Erika I.
Narváez-Padilla, Verónica
Reynaud, Enrique
author_facet Carvajal-Oliveros, Angel
Uriostegui-Arcos, Maritere
Zurita, Mario
Melchy-Perez, Erika I.
Narváez-Padilla, Verónica
Reynaud, Enrique
author_sort Carvajal-Oliveros, Angel
collection PubMed
description SH-SY5Y is a cell line derived from human neuroblastoma. It is one of the most widely used in vitro models to study Parkinson’s disease. Surprisingly, it has been found that it does not develop a dopaminergic phenotype after differentiation, questioning its usefulness as a Parkinson’s model. There are other in vitro models with better dopaminergic characteristics. BE (2)-M17 is a human neuroblastoma cell line that differentiates when treated with retinoic acid. We compared the dopaminergic and serotonergic properties of both cell lines. BE (2)-M17 has higher basal levels of dopaminergic markers and acquires a serotonergic phenotype during differentiation while maintaining the dopaminergic phenotype. SH-SY5Y has higher basal levels of serotonergic markers but does not acquire a dopaminergic phenotype upon differentiation.
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spelling pubmed-97189612022-12-04 The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic Carvajal-Oliveros, Angel Uriostegui-Arcos, Maritere Zurita, Mario Melchy-Perez, Erika I. Narváez-Padilla, Verónica Reynaud, Enrique IBRO Neurosci Rep Research Paper SH-SY5Y is a cell line derived from human neuroblastoma. It is one of the most widely used in vitro models to study Parkinson’s disease. Surprisingly, it has been found that it does not develop a dopaminergic phenotype after differentiation, questioning its usefulness as a Parkinson’s model. There are other in vitro models with better dopaminergic characteristics. BE (2)-M17 is a human neuroblastoma cell line that differentiates when treated with retinoic acid. We compared the dopaminergic and serotonergic properties of both cell lines. BE (2)-M17 has higher basal levels of dopaminergic markers and acquires a serotonergic phenotype during differentiation while maintaining the dopaminergic phenotype. SH-SY5Y has higher basal levels of serotonergic markers but does not acquire a dopaminergic phenotype upon differentiation. Elsevier 2022-11-20 /pmc/articles/PMC9718961/ /pubmed/36471713 http://dx.doi.org/10.1016/j.ibneur.2022.11.007 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Carvajal-Oliveros, Angel
Uriostegui-Arcos, Maritere
Zurita, Mario
Melchy-Perez, Erika I.
Narváez-Padilla, Verónica
Reynaud, Enrique
The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic
title The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic
title_full The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic
title_fullStr The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic
title_full_unstemmed The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic
title_short The BE (2)-M17 cell line has a better dopaminergic phenotype than the traditionally used for Parkinson´s research SH-SY5Y, which is mostly serotonergic
title_sort be (2)-m17 cell line has a better dopaminergic phenotype than the traditionally used for parkinson´s research sh-sy5y, which is mostly serotonergic
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718961/
https://www.ncbi.nlm.nih.gov/pubmed/36471713
http://dx.doi.org/10.1016/j.ibneur.2022.11.007
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