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Distinguishing parallel from series circuits with a double knockdown procedure in human cell lines
Signaling cascades can act in series or in parallel. Here, we describe a convenient and robust protocol for dual, sequential knockdown of two proteins using RNA interference. We detail the steps for a quantitative mapping of signaling circuitry. We used this approach to study kinases in human ovaria...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719107/ https://www.ncbi.nlm.nih.gov/pubmed/36595936 http://dx.doi.org/10.1016/j.xpro.2022.101890 |