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Chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats

BACKGROUND: Chlorogenic acid (CGA) has been shown to reduce pro-inflammation by scavenging reactive oxygen species (ROS) and reactive nitrogen species. In this study, the anti-inflammatory effect of CGA was expanded to streptozotocin (STZ)-induced diabetic rats. The inter-relationships among oxidati...

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Autores principales: Lee, Youngchan, Bae, Chun-Sik, Ahn, Taeho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719206/
https://www.ncbi.nlm.nih.gov/pubmed/36461118
http://dx.doi.org/10.1186/s42826-022-00148-x
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author Lee, Youngchan
Bae, Chun-Sik
Ahn, Taeho
author_facet Lee, Youngchan
Bae, Chun-Sik
Ahn, Taeho
author_sort Lee, Youngchan
collection PubMed
description BACKGROUND: Chlorogenic acid (CGA) has been shown to reduce pro-inflammation by scavenging reactive oxygen species (ROS) and reactive nitrogen species. In this study, the anti-inflammatory effect of CGA was expanded to streptozotocin (STZ)-induced diabetic rats. The inter-relationships among oxidative stress, pro-inflammation, and cytochrome P450 (CYP) 1A enzymes were also investigated in peripheral blood mononuclear cells (PBMC) of STZ-diabetic rats. RESULTS: The levels of pro-inflammatory cytokines, interleukin-6 and tumor necrosis factor-alpha, increased by approximately 3.4- and 2.9-fold, respectively, and the albumin concentration decreased in the serum of STZ-induced diabetic rats compared to normal rats. The C-reactive protein (CRP) values also increased by about 3.8-fold higher, indicating that STZ induced an inflammation in the blood of STZ-diabetic rats. The expression levels and catalytic activities of CYP1A enzymes were elevated by approximately 2.2–2.5- and 4.3–6.7-fold, respectively, in the PBMC of STZ-treated rats. A decrease in the amount of PBMC-bound albumin was also observed. In contrast, the levels of cytokines and CRP in serum and the activities of CYP1A enzymes in PBMC were significantly reduced in CGA-treated diabetic rats in a CGA concentration-dependent manner. In addition, STZ-mediated elevation of ROS in serum and PBMC was decreased by the CGA administration. However, the CGA treatment did not change the enhanced blood glucose level and expression of CYP1A enzymes by STZ. STZ-mediated decrease in the levels of serum and PBMC-bound albumin was not also restored by the CGA administration. CONCLUSIONS: These results suggest that CGA could be used to treat type 1 diabetes-induced inflammation.
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spelling pubmed-97192062022-12-04 Chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats Lee, Youngchan Bae, Chun-Sik Ahn, Taeho Lab Anim Res Research BACKGROUND: Chlorogenic acid (CGA) has been shown to reduce pro-inflammation by scavenging reactive oxygen species (ROS) and reactive nitrogen species. In this study, the anti-inflammatory effect of CGA was expanded to streptozotocin (STZ)-induced diabetic rats. The inter-relationships among oxidative stress, pro-inflammation, and cytochrome P450 (CYP) 1A enzymes were also investigated in peripheral blood mononuclear cells (PBMC) of STZ-diabetic rats. RESULTS: The levels of pro-inflammatory cytokines, interleukin-6 and tumor necrosis factor-alpha, increased by approximately 3.4- and 2.9-fold, respectively, and the albumin concentration decreased in the serum of STZ-induced diabetic rats compared to normal rats. The C-reactive protein (CRP) values also increased by about 3.8-fold higher, indicating that STZ induced an inflammation in the blood of STZ-diabetic rats. The expression levels and catalytic activities of CYP1A enzymes were elevated by approximately 2.2–2.5- and 4.3–6.7-fold, respectively, in the PBMC of STZ-treated rats. A decrease in the amount of PBMC-bound albumin was also observed. In contrast, the levels of cytokines and CRP in serum and the activities of CYP1A enzymes in PBMC were significantly reduced in CGA-treated diabetic rats in a CGA concentration-dependent manner. In addition, STZ-mediated elevation of ROS in serum and PBMC was decreased by the CGA administration. However, the CGA treatment did not change the enhanced blood glucose level and expression of CYP1A enzymes by STZ. STZ-mediated decrease in the levels of serum and PBMC-bound albumin was not also restored by the CGA administration. CONCLUSIONS: These results suggest that CGA could be used to treat type 1 diabetes-induced inflammation. BioMed Central 2022-12-02 /pmc/articles/PMC9719206/ /pubmed/36461118 http://dx.doi.org/10.1186/s42826-022-00148-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Lee, Youngchan
Bae, Chun-Sik
Ahn, Taeho
Chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats
title Chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats
title_full Chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats
title_fullStr Chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats
title_full_unstemmed Chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats
title_short Chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats
title_sort chlorogenic acid attenuates pro-inflammatory response in the blood of streptozotocin-induced diabetic rats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719206/
https://www.ncbi.nlm.nih.gov/pubmed/36461118
http://dx.doi.org/10.1186/s42826-022-00148-x
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