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The rate of microtubule breaking increases exponentially with curvature
Microtubules, cylindrical assemblies of tubulin proteins with a 25 nm diameter and micrometer lengths, are a central part of the cytoskeleton and also serve as building blocks for nanobiodevices. Microtubule breaking can result from the activity of severing enzymes and mechanical stress. Breaking ca...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719553/ https://www.ncbi.nlm.nih.gov/pubmed/36463258 http://dx.doi.org/10.1038/s41598-022-24912-0 |
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author | Tsitkov, Stanislav Rodriguez, Juan B. Bassir Kazeruni, Neda M. Sweet, May Nitta, Takahiro Hess, Henry |
author_facet | Tsitkov, Stanislav Rodriguez, Juan B. Bassir Kazeruni, Neda M. Sweet, May Nitta, Takahiro Hess, Henry |
author_sort | Tsitkov, Stanislav |
collection | PubMed |
description | Microtubules, cylindrical assemblies of tubulin proteins with a 25 nm diameter and micrometer lengths, are a central part of the cytoskeleton and also serve as building blocks for nanobiodevices. Microtubule breaking can result from the activity of severing enzymes and mechanical stress. Breaking can lead to a loss of structural integrity, or an increase in the numbers of microtubules. We observed breaking of taxol-stabilized microtubules in a gliding motility assay where microtubules are propelled by surface-adhered kinesin-1 motor proteins. We find that over 95% of all breaking events are associated with the strong bending following pinning events (where the leading tip of the microtubule becomes stuck). Furthermore, the breaking rate increased exponentially with increasing curvature. These observations are explained by a model accounting for the complex mechanochemistry of a microtubule. The presence of severing enzymes is not required to observe breaking at rates comparable to those measured previously in cells. |
format | Online Article Text |
id | pubmed-9719553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97195532022-12-05 The rate of microtubule breaking increases exponentially with curvature Tsitkov, Stanislav Rodriguez, Juan B. Bassir Kazeruni, Neda M. Sweet, May Nitta, Takahiro Hess, Henry Sci Rep Article Microtubules, cylindrical assemblies of tubulin proteins with a 25 nm diameter and micrometer lengths, are a central part of the cytoskeleton and also serve as building blocks for nanobiodevices. Microtubule breaking can result from the activity of severing enzymes and mechanical stress. Breaking can lead to a loss of structural integrity, or an increase in the numbers of microtubules. We observed breaking of taxol-stabilized microtubules in a gliding motility assay where microtubules are propelled by surface-adhered kinesin-1 motor proteins. We find that over 95% of all breaking events are associated with the strong bending following pinning events (where the leading tip of the microtubule becomes stuck). Furthermore, the breaking rate increased exponentially with increasing curvature. These observations are explained by a model accounting for the complex mechanochemistry of a microtubule. The presence of severing enzymes is not required to observe breaking at rates comparable to those measured previously in cells. Nature Publishing Group UK 2022-12-03 /pmc/articles/PMC9719553/ /pubmed/36463258 http://dx.doi.org/10.1038/s41598-022-24912-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Tsitkov, Stanislav Rodriguez, Juan B. Bassir Kazeruni, Neda M. Sweet, May Nitta, Takahiro Hess, Henry The rate of microtubule breaking increases exponentially with curvature |
title | The rate of microtubule breaking increases exponentially with curvature |
title_full | The rate of microtubule breaking increases exponentially with curvature |
title_fullStr | The rate of microtubule breaking increases exponentially with curvature |
title_full_unstemmed | The rate of microtubule breaking increases exponentially with curvature |
title_short | The rate of microtubule breaking increases exponentially with curvature |
title_sort | rate of microtubule breaking increases exponentially with curvature |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719553/ https://www.ncbi.nlm.nih.gov/pubmed/36463258 http://dx.doi.org/10.1038/s41598-022-24912-0 |
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