Cargando…
Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility
The binding of 17β-oestradiol to oestrogen receptor alpha (ERα) plays a crucial role in the control of reproduction, acting through both nuclear and membrane-initiated signalling. To study the physiological role of membrane ERα in the reproductive system, we used the C451A-ERα mouse model with selec...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9720743/ https://www.ncbi.nlm.nih.gov/pubmed/36239412 http://dx.doi.org/10.1242/dev.200683 |
_version_ | 1784843618212642816 |
---|---|
author | Rusidzé, Mariam Faure, Mélanie C. Sicard, Pierre Raymond-Letron, Isabelle Giton, Frank Vessieres, Emilie Prevot, Vincent Henrion, Daniel Arnal, Jean-François Cornil, Charlotte A. Lenfant, Françoise |
author_facet | Rusidzé, Mariam Faure, Mélanie C. Sicard, Pierre Raymond-Letron, Isabelle Giton, Frank Vessieres, Emilie Prevot, Vincent Henrion, Daniel Arnal, Jean-François Cornil, Charlotte A. Lenfant, Françoise |
author_sort | Rusidzé, Mariam |
collection | PubMed |
description | The binding of 17β-oestradiol to oestrogen receptor alpha (ERα) plays a crucial role in the control of reproduction, acting through both nuclear and membrane-initiated signalling. To study the physiological role of membrane ERα in the reproductive system, we used the C451A-ERα mouse model with selective loss of function of membrane ERα. Despite C451A-ERα mice being described as sterile, daily weighing and ultrasound imaging revealed that homozygous females do become pregnant, allowing the investigation of the role of ERα during pregnancy for the first time. All neonatal deaths of the mutant offspring mice resulted from delayed parturition associated with failure in pre-term progesterone withdrawal. Moreover, pregnant C451A-ERα females exhibited partial intrauterine embryo arrest at about E9.5. The observed embryonic lethality resulted from altered expansion of Tpbpa-positive spiral artery-associated trophoblast giant cells into the utero-placental unit, which is associated with an imbalance in expression of angiogenic factors. Together, these processes control the trophoblast-mediated spiral arterial remodelling. Hence, loss of membrane ERα within maternal tissues clearly alters the activity of invasive trophoblast cells during placentogenesis. This previously unreported function of membrane ERα could open new avenues towards a better understanding of human pregnancy-associated pathologies. |
format | Online Article Text |
id | pubmed-9720743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-97207432022-12-16 Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility Rusidzé, Mariam Faure, Mélanie C. Sicard, Pierre Raymond-Letron, Isabelle Giton, Frank Vessieres, Emilie Prevot, Vincent Henrion, Daniel Arnal, Jean-François Cornil, Charlotte A. Lenfant, Françoise Development Research Article The binding of 17β-oestradiol to oestrogen receptor alpha (ERα) plays a crucial role in the control of reproduction, acting through both nuclear and membrane-initiated signalling. To study the physiological role of membrane ERα in the reproductive system, we used the C451A-ERα mouse model with selective loss of function of membrane ERα. Despite C451A-ERα mice being described as sterile, daily weighing and ultrasound imaging revealed that homozygous females do become pregnant, allowing the investigation of the role of ERα during pregnancy for the first time. All neonatal deaths of the mutant offspring mice resulted from delayed parturition associated with failure in pre-term progesterone withdrawal. Moreover, pregnant C451A-ERα females exhibited partial intrauterine embryo arrest at about E9.5. The observed embryonic lethality resulted from altered expansion of Tpbpa-positive spiral artery-associated trophoblast giant cells into the utero-placental unit, which is associated with an imbalance in expression of angiogenic factors. Together, these processes control the trophoblast-mediated spiral arterial remodelling. Hence, loss of membrane ERα within maternal tissues clearly alters the activity of invasive trophoblast cells during placentogenesis. This previously unreported function of membrane ERα could open new avenues towards a better understanding of human pregnancy-associated pathologies. The Company of Biologists Ltd 2022-10-13 /pmc/articles/PMC9720743/ /pubmed/36239412 http://dx.doi.org/10.1242/dev.200683 Text en © 2022. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Rusidzé, Mariam Faure, Mélanie C. Sicard, Pierre Raymond-Letron, Isabelle Giton, Frank Vessieres, Emilie Prevot, Vincent Henrion, Daniel Arnal, Jean-François Cornil, Charlotte A. Lenfant, Françoise Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility |
title | Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility |
title_full | Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility |
title_fullStr | Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility |
title_full_unstemmed | Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility |
title_short | Loss of function of the maternal membrane oestrogen receptor ERα alters expansion of trophoblast cells and impacts mouse fertility |
title_sort | loss of function of the maternal membrane oestrogen receptor erα alters expansion of trophoblast cells and impacts mouse fertility |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9720743/ https://www.ncbi.nlm.nih.gov/pubmed/36239412 http://dx.doi.org/10.1242/dev.200683 |
work_keys_str_mv | AT rusidzemariam lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT fauremelaniec lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT sicardpierre lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT raymondletronisabelle lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT gitonfrank lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT vessieresemilie lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT prevotvincent lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT henriondaniel lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT arnaljeanfrancois lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT cornilcharlottea lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility AT lenfantfrancoise lossoffunctionofthematernalmembraneoestrogenreceptoreraaltersexpansionoftrophoblastcellsandimpactsmousefertility |