Cargando…

Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4

Ashy stem blight (ASB), caused by the fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease of the common bean (Phaseolus vulgaris L.). It is important to identify quantitative trait loci (QTL) for ASB resistance and introgress into susceptible cultivars of the common bean. The ob...

Descripción completa

Detalles Bibliográficos
Autores principales: Viteri, Diego M., Linares, Angela M., Miranda, Zoralys, Shi, Ainong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9721262/
https://www.ncbi.nlm.nih.gov/pubmed/36479519
http://dx.doi.org/10.3389/fpls.2022.1019263
_version_ 1784843736203657216
author Viteri, Diego M.
Linares, Angela M.
Miranda, Zoralys
Shi, Ainong
author_facet Viteri, Diego M.
Linares, Angela M.
Miranda, Zoralys
Shi, Ainong
author_sort Viteri, Diego M.
collection PubMed
description Ashy stem blight (ASB), caused by the fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease of the common bean (Phaseolus vulgaris L.). It is important to identify quantitative trait loci (QTL) for ASB resistance and introgress into susceptible cultivars of the common bean. The objective of this research was to identify QTL and single nucleotide polymorphism (SNP) markers associated with ASB resistance in recombinant inbred lines (RIL) derived from a cross between BAT 477 and NY6020-4 common bean. One hundred and twenty-six F(6:7) RIL were phenotyped for ASB in the greenhouse. Disease severity was scored on a scale of 1–9. Genotyping was performed using whole genome resequencing with 2x common bean genome size coverage, and over six million SNPs were obtained. After being filtered, 72,017 SNPs distributed on 11 chromosomes were used to conduct the genome-wide association study (GWAS) and QTL mapping. A novel QTL region of ~4.28 Mbp from 35,546,329 bp to 39,826,434 bp on chromosome Pv03 was identified for ASB resistance. The two SNPs, Chr03_39824257 and Chr03_39824268 located at 39,824,257 bp and 39,824,268 bp on Pv03, respectively, were identified as the strongest markers associated with ASB resistance. The gene Phvul.003G175900 (drought sensitive, WD repeat-containing protein 76) located at 39,822,021 – 39,824,655 bp on Pv03 was recognized as one candidate for ASB resistance in the RIL, and the gene contained the two SNP markers. QTL and SNP markers may be used to select plants and lines for ASB resistance through marker-assisted selection (MAS) in common bean breeding.
format Online
Article
Text
id pubmed-9721262
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97212622022-12-06 Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4 Viteri, Diego M. Linares, Angela M. Miranda, Zoralys Shi, Ainong Front Plant Sci Plant Science Ashy stem blight (ASB), caused by the fungus Macrophomina phaseolina (Tassi) Goidanich is an important disease of the common bean (Phaseolus vulgaris L.). It is important to identify quantitative trait loci (QTL) for ASB resistance and introgress into susceptible cultivars of the common bean. The objective of this research was to identify QTL and single nucleotide polymorphism (SNP) markers associated with ASB resistance in recombinant inbred lines (RIL) derived from a cross between BAT 477 and NY6020-4 common bean. One hundred and twenty-six F(6:7) RIL were phenotyped for ASB in the greenhouse. Disease severity was scored on a scale of 1–9. Genotyping was performed using whole genome resequencing with 2x common bean genome size coverage, and over six million SNPs were obtained. After being filtered, 72,017 SNPs distributed on 11 chromosomes were used to conduct the genome-wide association study (GWAS) and QTL mapping. A novel QTL region of ~4.28 Mbp from 35,546,329 bp to 39,826,434 bp on chromosome Pv03 was identified for ASB resistance. The two SNPs, Chr03_39824257 and Chr03_39824268 located at 39,824,257 bp and 39,824,268 bp on Pv03, respectively, were identified as the strongest markers associated with ASB resistance. The gene Phvul.003G175900 (drought sensitive, WD repeat-containing protein 76) located at 39,822,021 – 39,824,655 bp on Pv03 was recognized as one candidate for ASB resistance in the RIL, and the gene contained the two SNP markers. QTL and SNP markers may be used to select plants and lines for ASB resistance through marker-assisted selection (MAS) in common bean breeding. Frontiers Media S.A. 2022-11-21 /pmc/articles/PMC9721262/ /pubmed/36479519 http://dx.doi.org/10.3389/fpls.2022.1019263 Text en Copyright © 2022 Viteri, Linares, Miranda and Shi https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Viteri, Diego M.
Linares, Angela M.
Miranda, Zoralys
Shi, Ainong
Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4
title Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4
title_full Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4
title_fullStr Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4
title_full_unstemmed Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4
title_short Identification of a QTL region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from BAT 477 and NY6020-4
title_sort identification of a qtl region for ashy stem blight resistance using genome-wide association and linage analysis in common bean recombinant inbred lines derived from bat 477 and ny6020-4
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9721262/
https://www.ncbi.nlm.nih.gov/pubmed/36479519
http://dx.doi.org/10.3389/fpls.2022.1019263
work_keys_str_mv AT viteridiegom identificationofaqtlregionforashystemblightresistanceusinggenomewideassociationandlinageanalysisincommonbeanrecombinantinbredlinesderivedfrombat477andny60204
AT linaresangelam identificationofaqtlregionforashystemblightresistanceusinggenomewideassociationandlinageanalysisincommonbeanrecombinantinbredlinesderivedfrombat477andny60204
AT mirandazoralys identificationofaqtlregionforashystemblightresistanceusinggenomewideassociationandlinageanalysisincommonbeanrecombinantinbredlinesderivedfrombat477andny60204
AT shiainong identificationofaqtlregionforashystemblightresistanceusinggenomewideassociationandlinageanalysisincommonbeanrecombinantinbredlinesderivedfrombat477andny60204