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Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections

Small-angle X-ray scattering (SAXS) from fibrils embedded in a fixed, thin section of tissue includes contributions from the fibrils, the polymeric matrix surrounding the fibrils, other constituents of the tissue, and cross-terms due to the spatial correlation between fibrils and neighboring molecul...

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Autores principales: Nepal, Prakash, Al Bashit, Abdullah, Yang, Lin, Makowski, Lee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9721334/
https://www.ncbi.nlm.nih.gov/pubmed/36570653
http://dx.doi.org/10.1107/S1600576722009955
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author Nepal, Prakash
Al Bashit, Abdullah
Yang, Lin
Makowski, Lee
author_facet Nepal, Prakash
Al Bashit, Abdullah
Yang, Lin
Makowski, Lee
author_sort Nepal, Prakash
collection PubMed
description Small-angle X-ray scattering (SAXS) from fibrils embedded in a fixed, thin section of tissue includes contributions from the fibrils, the polymeric matrix surrounding the fibrils, other constituents of the tissue, and cross-terms due to the spatial correlation between fibrils and neighboring molecules. This complex mixture severely limits the amount of information that can be extracted from scattering studies. However, availability of micro- and nano-beams has made the measurement of scattering from very small volumes possible, which, in some cases, may be dominated by a single fibrillar constituent. In such cases, information about the predominant species may be accessible. Nevertheless, even in these cases, the correlations between the positions of fibrils and other constituents have a significant impact on the observed scattering. Here, strategies are proposed to extract partial information about fibril structure and tissue organization on the basis of SAXS from samples of this type. It is shown that the spatial correlation function of the fibril in the direction perpendicular to the fibril axis can be computed and contains information about the predominant fibril structure and the organization of the surrounding tissue matrix. This has significant advantages over approaches based on techniques developed for X-ray solution scattering. Examples of correlation calculations in different types of samples are given to demonstrate the information that can be obtained from these measurements.
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spelling pubmed-97213342022-12-22 Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections Nepal, Prakash Al Bashit, Abdullah Yang, Lin Makowski, Lee J Appl Crystallogr Research Papers Small-angle X-ray scattering (SAXS) from fibrils embedded in a fixed, thin section of tissue includes contributions from the fibrils, the polymeric matrix surrounding the fibrils, other constituents of the tissue, and cross-terms due to the spatial correlation between fibrils and neighboring molecules. This complex mixture severely limits the amount of information that can be extracted from scattering studies. However, availability of micro- and nano-beams has made the measurement of scattering from very small volumes possible, which, in some cases, may be dominated by a single fibrillar constituent. In such cases, information about the predominant species may be accessible. Nevertheless, even in these cases, the correlations between the positions of fibrils and other constituents have a significant impact on the observed scattering. Here, strategies are proposed to extract partial information about fibril structure and tissue organization on the basis of SAXS from samples of this type. It is shown that the spatial correlation function of the fibril in the direction perpendicular to the fibril axis can be computed and contains information about the predominant fibril structure and the organization of the surrounding tissue matrix. This has significant advantages over approaches based on techniques developed for X-ray solution scattering. Examples of correlation calculations in different types of samples are given to demonstrate the information that can be obtained from these measurements. International Union of Crystallography 2022-11-21 /pmc/articles/PMC9721334/ /pubmed/36570653 http://dx.doi.org/10.1107/S1600576722009955 Text en © Prakash Nepal et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Nepal, Prakash
Al Bashit, Abdullah
Yang, Lin
Makowski, Lee
Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections
title Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections
title_full Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections
title_fullStr Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections
title_full_unstemmed Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections
title_short Small-angle X-ray microdiffraction from fibrils embedded in tissue thin sections
title_sort small-angle x-ray microdiffraction from fibrils embedded in tissue thin sections
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9721334/
https://www.ncbi.nlm.nih.gov/pubmed/36570653
http://dx.doi.org/10.1107/S1600576722009955
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