Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome

The isolation of chondrocytes from human articular cartilage for single-cell RNA sequencing requires extensive and prolonged tissue digestion at 37 C. Modulations of the transcriptional activity likely take place during this period such that the transcriptomes of isolated human chondrocytes no longe...

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Detalles Bibliográficos
Autores principales: Shen, Ping, Wu, Peihua, Maleitzke, Tazio, Reisener, Marie-Jacqueline, Heinz, Gitta A., Heinrich, Frederik, Durek, Pawel, Gwinner, Clemens, Winkler, Tobias, Pumberger, Matthias, Perka, Carsten, Mashreghi, Mir-Farzin, Löhning, Max
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9721448/
https://www.ncbi.nlm.nih.gov/pubmed/36479429
http://dx.doi.org/10.3389/fbioe.2022.1046127

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