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Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp

Muscle larva of the parasitic nematode Trichinella spp. lives in a portion of muscle fibre transformed to a nurse cell (NC). Based on our previous transcriptomic studies, NC growth arrest was inferred to be accompanied by cellular senescence. In the current study, NC was proven to display the follow...

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Autores principales: Dabrowska, Magdalena, Kępczyńska, Agnieszka, Goździk, Katarzyna, Całka-Kresa, Małgorzata, Skoneczny, Marek, Zieliński, Zbigniew, Doligalska, Maria, Sikora, Ewa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722307/
https://www.ncbi.nlm.nih.gov/pubmed/36478989
http://dx.doi.org/10.1155/2022/1799839
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author Dabrowska, Magdalena
Kępczyńska, Agnieszka
Goździk, Katarzyna
Całka-Kresa, Małgorzata
Skoneczny, Marek
Zieliński, Zbigniew
Doligalska, Maria
Sikora, Ewa
author_facet Dabrowska, Magdalena
Kępczyńska, Agnieszka
Goździk, Katarzyna
Całka-Kresa, Małgorzata
Skoneczny, Marek
Zieliński, Zbigniew
Doligalska, Maria
Sikora, Ewa
author_sort Dabrowska, Magdalena
collection PubMed
description Muscle larva of the parasitic nematode Trichinella spp. lives in a portion of muscle fibre transformed to a nurse cell (NC). Based on our previous transcriptomic studies, NC growth arrest was inferred to be accompanied by cellular senescence. In the current study, NC was proven to display the following markers of senescence: high senescence-associated β-galactosidase activity, lipid deposition, DNA damage, and cell cycle inhibition. Moreover, the nuclear localization of Activator Protein 1 (c-Fos, c-Jun, and FosB), as well as the upregulation of numerous AP-1 target genes in the NC, remained in accord with AP-1 recently identified as a master transcription factor in senescence. An increase in reactive oxygen species generation and the upregulation of antioxidant defence enzymes, including glutathione peroxidases 1 and 3, catalase, superoxide dismutases 1 and 3, and heme oxygenase 1, indicated an ongoing oxidative stress to proceed in the NC. Interestingly, antioxidant defence enzymes localized not only to the NC but also to the larva. These results allowed us to hypothesize that oxidative stress accompanying muscle regeneration and larval antigenic properties lead to the transformation of a regenerating myofibre into a senescent cell. Cellular senescence apparently represents a state of metabolism that sustains the long-term existence of muscle larva and ultimately provides it with the antioxidant capacity needed during the next host colonization. Senotherapy, a therapeutic approach aimed at selective elimination of senescent cells, can thus be viewed as potentially effective in the treatment of trichinosis.
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spelling pubmed-97223072022-12-06 Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp Dabrowska, Magdalena Kępczyńska, Agnieszka Goździk, Katarzyna Całka-Kresa, Małgorzata Skoneczny, Marek Zieliński, Zbigniew Doligalska, Maria Sikora, Ewa Oxid Med Cell Longev Research Article Muscle larva of the parasitic nematode Trichinella spp. lives in a portion of muscle fibre transformed to a nurse cell (NC). Based on our previous transcriptomic studies, NC growth arrest was inferred to be accompanied by cellular senescence. In the current study, NC was proven to display the following markers of senescence: high senescence-associated β-galactosidase activity, lipid deposition, DNA damage, and cell cycle inhibition. Moreover, the nuclear localization of Activator Protein 1 (c-Fos, c-Jun, and FosB), as well as the upregulation of numerous AP-1 target genes in the NC, remained in accord with AP-1 recently identified as a master transcription factor in senescence. An increase in reactive oxygen species generation and the upregulation of antioxidant defence enzymes, including glutathione peroxidases 1 and 3, catalase, superoxide dismutases 1 and 3, and heme oxygenase 1, indicated an ongoing oxidative stress to proceed in the NC. Interestingly, antioxidant defence enzymes localized not only to the NC but also to the larva. These results allowed us to hypothesize that oxidative stress accompanying muscle regeneration and larval antigenic properties lead to the transformation of a regenerating myofibre into a senescent cell. Cellular senescence apparently represents a state of metabolism that sustains the long-term existence of muscle larva and ultimately provides it with the antioxidant capacity needed during the next host colonization. Senotherapy, a therapeutic approach aimed at selective elimination of senescent cells, can thus be viewed as potentially effective in the treatment of trichinosis. Hindawi 2022-11-28 /pmc/articles/PMC9722307/ /pubmed/36478989 http://dx.doi.org/10.1155/2022/1799839 Text en Copyright © 2022 Magdalena Dabrowska et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dabrowska, Magdalena
Kępczyńska, Agnieszka
Goździk, Katarzyna
Całka-Kresa, Małgorzata
Skoneczny, Marek
Zieliński, Zbigniew
Doligalska, Maria
Sikora, Ewa
Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp
title Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp
title_full Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp
title_fullStr Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp
title_full_unstemmed Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp
title_short Novel Role of Mammalian Cell Senescence-Sustenance of Muscle Larvae of Trichinella spp
title_sort novel role of mammalian cell senescence-sustenance of muscle larvae of trichinella spp
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722307/
https://www.ncbi.nlm.nih.gov/pubmed/36478989
http://dx.doi.org/10.1155/2022/1799839
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