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Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts

Demineralized bone matrix (DBM) is considered one of the most reliable bone tissue grafts for regular surgical use, as it provides a scaffold that is structurally like native bone, and that enhances bone regeneration. However, commercially available DBM products are not suited for surgical restituti...

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Autores principales: Jaramillo-Cañas, Winston, Britto-Bisso, Frank, Fernandez-Valiente, Cesar, Casado, Fanny L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722481/
https://www.ncbi.nlm.nih.gov/pubmed/36483326
http://dx.doi.org/10.1016/j.ohx.2022.e00378
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author Jaramillo-Cañas, Winston
Britto-Bisso, Frank
Fernandez-Valiente, Cesar
Casado, Fanny L.
author_facet Jaramillo-Cañas, Winston
Britto-Bisso, Frank
Fernandez-Valiente, Cesar
Casado, Fanny L.
author_sort Jaramillo-Cañas, Winston
collection PubMed
description Demineralized bone matrix (DBM) is considered one of the most reliable bone tissue grafts for regular surgical use, as it provides a scaffold that is structurally like native bone, and that enhances bone regeneration. However, commercially available DBM products are not suited for surgical restitutions of large bones. Therefore, each Tissue Bank is urged to implement their own demineralization protocol, which usually does not meet the high demand for bone grafting. In this project, we developed an open source system for medium-scale manufacturing of DBM grafts from human cadaveric donors to automate the demineralization protocol and improve its reproducibility. The device consists in (1) unidirectional flow reaction chamber, where the demineralization protocol takes place; (2) automated syringe pump, which controls the reagent́s inlet and vacuum; and (3) reagent dispenser, for the management of the reagents need for the demineralization protocol. Validation of the device included histological analysis, DNA quantification temperature regulation, electrochemiluminescence and colorimetric protocols, followed by the optimization of physicochemical parameters based on Response Surface Methodology. The results showed values of residual lipids and calcium within standardized ranges, and the maintenance of the structural integrity of the DBM, demonstrating the capacity of the system to support the proposed demineralization protocol.
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spelling pubmed-97224812022-12-07 Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts Jaramillo-Cañas, Winston Britto-Bisso, Frank Fernandez-Valiente, Cesar Casado, Fanny L. HardwareX Article Demineralized bone matrix (DBM) is considered one of the most reliable bone tissue grafts for regular surgical use, as it provides a scaffold that is structurally like native bone, and that enhances bone regeneration. However, commercially available DBM products are not suited for surgical restitutions of large bones. Therefore, each Tissue Bank is urged to implement their own demineralization protocol, which usually does not meet the high demand for bone grafting. In this project, we developed an open source system for medium-scale manufacturing of DBM grafts from human cadaveric donors to automate the demineralization protocol and improve its reproducibility. The device consists in (1) unidirectional flow reaction chamber, where the demineralization protocol takes place; (2) automated syringe pump, which controls the reagent́s inlet and vacuum; and (3) reagent dispenser, for the management of the reagents need for the demineralization protocol. Validation of the device included histological analysis, DNA quantification temperature regulation, electrochemiluminescence and colorimetric protocols, followed by the optimization of physicochemical parameters based on Response Surface Methodology. The results showed values of residual lipids and calcium within standardized ranges, and the maintenance of the structural integrity of the DBM, demonstrating the capacity of the system to support the proposed demineralization protocol. Elsevier 2022-11-23 /pmc/articles/PMC9722481/ /pubmed/36483326 http://dx.doi.org/10.1016/j.ohx.2022.e00378 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jaramillo-Cañas, Winston
Britto-Bisso, Frank
Fernandez-Valiente, Cesar
Casado, Fanny L.
Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts
title Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts
title_full Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts
title_fullStr Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts
title_full_unstemmed Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts
title_short Open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts
title_sort open-source perfusion system for medium-scale fabrication of demineralized bone matrix chip grafts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722481/
https://www.ncbi.nlm.nih.gov/pubmed/36483326
http://dx.doi.org/10.1016/j.ohx.2022.e00378
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