Protocol to assess RNA-RNA interactions in situ using an RNA-proximity ligation assay

Here, we describe a protocol to assess RNA-RNA interactions in situ using an adapted proximity ligation assay (PLA). We detail steps to perform RNA-probe hybridization, in situ rolling circle amplification, and immunofluorescence confocal microscopy. With these tools, it is possible to detect and ch...

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Detalles Bibliográficos
Autores principales: Basavappa, Megha G., Henao-Mejia, Jorge, Cherry, Sara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722720/
https://www.ncbi.nlm.nih.gov/pubmed/36595913
http://dx.doi.org/10.1016/j.xpro.2022.101892

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