Protocol for recording and analyzing spontaneous locomotion in Drosophila

The quantitative analysis of locomotion is used to study many biological processes. Here, we describe how to record the locomotion of up to 50 Drosophila individuals and process the resulting video files using FlyTracker. We detail the use of modifiable MatLab scripts to process structure array file...

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Detalles Bibliográficos
Autores principales: Barwell, Taylor, Raina, Sehaj, Seroude, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722781/
https://www.ncbi.nlm.nih.gov/pubmed/36595964
http://dx.doi.org/10.1016/j.xpro.2022.101888
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author Barwell, Taylor
Raina, Sehaj
Seroude, Laurent
author_facet Barwell, Taylor
Raina, Sehaj
Seroude, Laurent
author_sort Barwell, Taylor
collection PubMed
description The quantitative analysis of locomotion is used to study many biological processes. Here, we describe how to record the locomotion of up to 50 Drosophila individuals and process the resulting video files using FlyTracker. We detail the use of modifiable MatLab scripts to process structure array files generated by FlyTracker. We have applied this to study Drosophila movement during aging, but it could be used to address a variety of research questions. For complete details on the use and execution of this protocol, please refer to Barwell et al. (2021).(1)
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spelling pubmed-97227812022-12-07 Protocol for recording and analyzing spontaneous locomotion in Drosophila Barwell, Taylor Raina, Sehaj Seroude, Laurent STAR Protoc Protocol The quantitative analysis of locomotion is used to study many biological processes. Here, we describe how to record the locomotion of up to 50 Drosophila individuals and process the resulting video files using FlyTracker. We detail the use of modifiable MatLab scripts to process structure array files generated by FlyTracker. We have applied this to study Drosophila movement during aging, but it could be used to address a variety of research questions. For complete details on the use and execution of this protocol, please refer to Barwell et al. (2021).(1) Elsevier 2022-12-02 /pmc/articles/PMC9722781/ /pubmed/36595964 http://dx.doi.org/10.1016/j.xpro.2022.101888 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Barwell, Taylor
Raina, Sehaj
Seroude, Laurent
Protocol for recording and analyzing spontaneous locomotion in Drosophila
title Protocol for recording and analyzing spontaneous locomotion in Drosophila
title_full Protocol for recording and analyzing spontaneous locomotion in Drosophila
title_fullStr Protocol for recording and analyzing spontaneous locomotion in Drosophila
title_full_unstemmed Protocol for recording and analyzing spontaneous locomotion in Drosophila
title_short Protocol for recording and analyzing spontaneous locomotion in Drosophila
title_sort protocol for recording and analyzing spontaneous locomotion in drosophila
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722781/
https://www.ncbi.nlm.nih.gov/pubmed/36595964
http://dx.doi.org/10.1016/j.xpro.2022.101888
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