In vitro and in cellulo ApoE particle formation, isolation, and characterization

Apolipoprotein E (ApoE) particles are responsible for packing and transporting lipids throughout aqueous environments. We detail steps to assess in vitro particles forming from artificial membranes using right-angle light scattering and to measure their size using dynamic light scattering. We furthe...

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Autores principales: Lindner, Karina, van Ek, Larissa C., Awwad, Khader, Hanke, Franziska, Korepanova, Alla V., Rybin, Vladimir, Beckenbauer, Katharina, Gavin, Anne-Claude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722783/
https://www.ncbi.nlm.nih.gov/pubmed/36595894
http://dx.doi.org/10.1016/j.xpro.2022.101894
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author Lindner, Karina
van Ek, Larissa C.
Awwad, Khader
Hanke, Franziska
Korepanova, Alla V.
Rybin, Vladimir
Beckenbauer, Katharina
Gavin, Anne-Claude
author_facet Lindner, Karina
van Ek, Larissa C.
Awwad, Khader
Hanke, Franziska
Korepanova, Alla V.
Rybin, Vladimir
Beckenbauer, Katharina
Gavin, Anne-Claude
author_sort Lindner, Karina
collection PubMed
description Apolipoprotein E (ApoE) particles are responsible for packing and transporting lipids throughout aqueous environments. We detail steps to assess in vitro particles forming from artificial membranes using right-angle light scattering and to measure their size using dynamic light scattering. We further describe how to generate in cellulo ApoE particles containing triacylglycerol under fatty-acid-induced stress. We also detail steps to isolate them from cell secretome by immunoprecipitation and analyze their lipid cargo by thin-layer chromatography. For complete details on the use and execution of this protocol, please refer to Lindner et al. (2022).(1)
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spelling pubmed-97227832022-12-07 In vitro and in cellulo ApoE particle formation, isolation, and characterization Lindner, Karina van Ek, Larissa C. Awwad, Khader Hanke, Franziska Korepanova, Alla V. Rybin, Vladimir Beckenbauer, Katharina Gavin, Anne-Claude STAR Protoc Protocol Apolipoprotein E (ApoE) particles are responsible for packing and transporting lipids throughout aqueous environments. We detail steps to assess in vitro particles forming from artificial membranes using right-angle light scattering and to measure their size using dynamic light scattering. We further describe how to generate in cellulo ApoE particles containing triacylglycerol under fatty-acid-induced stress. We also detail steps to isolate them from cell secretome by immunoprecipitation and analyze their lipid cargo by thin-layer chromatography. For complete details on the use and execution of this protocol, please refer to Lindner et al. (2022).(1) Elsevier 2022-12-02 /pmc/articles/PMC9722783/ /pubmed/36595894 http://dx.doi.org/10.1016/j.xpro.2022.101894 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lindner, Karina
van Ek, Larissa C.
Awwad, Khader
Hanke, Franziska
Korepanova, Alla V.
Rybin, Vladimir
Beckenbauer, Katharina
Gavin, Anne-Claude
In vitro and in cellulo ApoE particle formation, isolation, and characterization
title In vitro and in cellulo ApoE particle formation, isolation, and characterization
title_full In vitro and in cellulo ApoE particle formation, isolation, and characterization
title_fullStr In vitro and in cellulo ApoE particle formation, isolation, and characterization
title_full_unstemmed In vitro and in cellulo ApoE particle formation, isolation, and characterization
title_short In vitro and in cellulo ApoE particle formation, isolation, and characterization
title_sort in vitro and in cellulo apoe particle formation, isolation, and characterization
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9722783/
https://www.ncbi.nlm.nih.gov/pubmed/36595894
http://dx.doi.org/10.1016/j.xpro.2022.101894
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