The Effect of Bleaching Agents on the Microstructure and Surface Microhardness of Three Calcium Silicate-Based Barrier Materials

INTRODUCTION: The aim of this study was to evaluate the effects of exposure to sodium perborate and H(2)O(2) on the surface characteristics of MTA Angelus, Biodentine and MTA Repair HP after 1 and 6 month time intervals. METHODS AND MATERIALS: Three calcium silicate-based cements were evaluated: MTA Angelus, Biodentine, MTA Repair HP. A total of 234 specimens were stored in Hank’s balanced salt solution (HBSS) for 1 month or 6 months in which afterwards were divided into 3 groups according to bleaching agent applied: control, sodium perborate, 35% hydrogen peroxide. The microstructural changes were evaluated by scanning electron microscopy and energy-dispersive X-ray spectroscopy. The surface microhardness was also evaluated. Data were analyzed by one-way analysis of variance and Games-Howell post-hoc tests for the effect bleaching agents and hydraulic calcium silicate-based cements and t-test was for the effect of time. RESULTS: Distinctive alterations with uneven depression areas, woodpecker defects and cracks were seen due to exposure to perborate and H(2)O(2) on all evaluated cements. Exposure to H(2)O(2) caused a decrease in Ca/Si ratio in all experimental cements. Both H(2)O(2) and perborate significantly decreased the microhardness of all cement (P<0.05) with H(2)O(2) having a more profound effect (P<0.01). A 6-month delay in exposure to bleaching agents significantly increased the microhardness of Biodentine compared to 1 month (P<0.001 for both bleaching agents). CONCLUSION: Based on this in vitro study, H(2)O(2) had more detrimental effects on MTA Angelus, Biodentine and MTA Repair HP. Sodium perborate may be a more conservative selection when considering effects on barrier materials.