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Nanocellulose coated paper diagnostic to measure glucose concentration in human blood

A new generation of rapid, easy to use and robust colorimetric point of care (POC) nanocellulose coated-paper sensors to measure glucose concentration in blood is presented in this study. The cellulose gel containing the enzyme with co-additive is coated and dried onto a paper substrate. Nanocellulo...

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Autores principales: Hossain, Laila, De Francesco, Marisa, Tedja, Patricia, Tanner, Joanne, Garnier, Gil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723229/
https://www.ncbi.nlm.nih.gov/pubmed/36483768
http://dx.doi.org/10.3389/fbioe.2022.1052242
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author Hossain, Laila
De Francesco, Marisa
Tedja, Patricia
Tanner, Joanne
Garnier, Gil
author_facet Hossain, Laila
De Francesco, Marisa
Tedja, Patricia
Tanner, Joanne
Garnier, Gil
author_sort Hossain, Laila
collection PubMed
description A new generation of rapid, easy to use and robust colorimetric point of care (POC) nanocellulose coated-paper sensors to measure glucose concentration in blood is presented in this study. The cellulose gel containing the enzyme with co-additive is coated and dried onto a paper substrate. Nanocellulose gel is used to store, immobilize and stabilize enzymes within its structure to prolong enzyme function and enhance its availability. Here, we immobilize glucose oxidase within the gel structure to produce a simple colorimetric blood glucose sensor. Increase in blood glucose concentration increases the concentration of reaction product which decreases the system pH detected by the pH indicative dye entrapped in the nanocellulose gel. The sensor produces a color change from red to orange as pH decreases due to the enzymatic reaction of glucose into gluconic acid and hydrogen peroxide. This sensor can measure glucose concentrations of 7–13 mM (medical range for diabetes control) at temperatures of 4°C–40°C. Stability tests confirm that no denaturation of enzyme occurs by measuring enzyme activity after 4 weeks. A prototype device is designed to instantly measure the glucose concentration from blood in a two steps process: 1) red blood cell separation and 2) quantification of glucose by color change. This study demonstrates nanocellulose sensor as an economical, robust, and sensitive diagnostic technology platform for a broad spectrum of diseases.
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spelling pubmed-97232292022-12-07 Nanocellulose coated paper diagnostic to measure glucose concentration in human blood Hossain, Laila De Francesco, Marisa Tedja, Patricia Tanner, Joanne Garnier, Gil Front Bioeng Biotechnol Bioengineering and Biotechnology A new generation of rapid, easy to use and robust colorimetric point of care (POC) nanocellulose coated-paper sensors to measure glucose concentration in blood is presented in this study. The cellulose gel containing the enzyme with co-additive is coated and dried onto a paper substrate. Nanocellulose gel is used to store, immobilize and stabilize enzymes within its structure to prolong enzyme function and enhance its availability. Here, we immobilize glucose oxidase within the gel structure to produce a simple colorimetric blood glucose sensor. Increase in blood glucose concentration increases the concentration of reaction product which decreases the system pH detected by the pH indicative dye entrapped in the nanocellulose gel. The sensor produces a color change from red to orange as pH decreases due to the enzymatic reaction of glucose into gluconic acid and hydrogen peroxide. This sensor can measure glucose concentrations of 7–13 mM (medical range for diabetes control) at temperatures of 4°C–40°C. Stability tests confirm that no denaturation of enzyme occurs by measuring enzyme activity after 4 weeks. A prototype device is designed to instantly measure the glucose concentration from blood in a two steps process: 1) red blood cell separation and 2) quantification of glucose by color change. This study demonstrates nanocellulose sensor as an economical, robust, and sensitive diagnostic technology platform for a broad spectrum of diseases. Frontiers Media S.A. 2022-11-22 /pmc/articles/PMC9723229/ /pubmed/36483768 http://dx.doi.org/10.3389/fbioe.2022.1052242 Text en Copyright © 2022 Hossain, De Francesco, Tedja, Tanner and Garnier. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Hossain, Laila
De Francesco, Marisa
Tedja, Patricia
Tanner, Joanne
Garnier, Gil
Nanocellulose coated paper diagnostic to measure glucose concentration in human blood
title Nanocellulose coated paper diagnostic to measure glucose concentration in human blood
title_full Nanocellulose coated paper diagnostic to measure glucose concentration in human blood
title_fullStr Nanocellulose coated paper diagnostic to measure glucose concentration in human blood
title_full_unstemmed Nanocellulose coated paper diagnostic to measure glucose concentration in human blood
title_short Nanocellulose coated paper diagnostic to measure glucose concentration in human blood
title_sort nanocellulose coated paper diagnostic to measure glucose concentration in human blood
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723229/
https://www.ncbi.nlm.nih.gov/pubmed/36483768
http://dx.doi.org/10.3389/fbioe.2022.1052242
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