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Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions

Bone tissue features a complex microarchitecture and biomolecular composition, which determine biomechanical properties. In addition to state-of-the-art technologies, innovative optical approaches allowing the characterization of the bone in native, label-free conditions can provide new, multi-level...

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Autores principales: Talone, Benedetta, Bresci, Arianna, Manetti, Francesco, Vernuccio, Federico, De la Cadena, Alejandro, Ceconello, Chiara, Schiavone, Maria Lucia, Mantero, Stefano, Menale, Ciro, Vanna, Renzo, Cerullo, Giulio, Sobacchi, Cristina, Polli, Dario
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723390/
https://www.ncbi.nlm.nih.gov/pubmed/36483771
http://dx.doi.org/10.3389/fbioe.2022.1042680
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author Talone, Benedetta
Bresci, Arianna
Manetti, Francesco
Vernuccio, Federico
De la Cadena, Alejandro
Ceconello, Chiara
Schiavone, Maria Lucia
Mantero, Stefano
Menale, Ciro
Vanna, Renzo
Cerullo, Giulio
Sobacchi, Cristina
Polli, Dario
author_facet Talone, Benedetta
Bresci, Arianna
Manetti, Francesco
Vernuccio, Federico
De la Cadena, Alejandro
Ceconello, Chiara
Schiavone, Maria Lucia
Mantero, Stefano
Menale, Ciro
Vanna, Renzo
Cerullo, Giulio
Sobacchi, Cristina
Polli, Dario
author_sort Talone, Benedetta
collection PubMed
description Bone tissue features a complex microarchitecture and biomolecular composition, which determine biomechanical properties. In addition to state-of-the-art technologies, innovative optical approaches allowing the characterization of the bone in native, label-free conditions can provide new, multi-level insight into this inherently challenging tissue. Here, we exploited multimodal nonlinear optical (NLO) microscopy, including co-registered stimulated Raman scattering, two-photon excited fluorescence, and second-harmonic generation, to image entire vertebrae of murine spine sections. The quantitative nature of these nonlinear interactions allowed us to extract accurate biochemical, morphological, and topological information on the bone tissue and to highlight differences between normal and pathologic samples. Indeed, in a murine model showing bone loss, we observed increased collagen and lipid content as compared to the wild type, along with a decreased craniocaudal alignment of bone collagen fibres. We propose that NLO microscopy can be implemented in standard histopathological analysis of bone in preclinical studies, with the ambitious future perspective to introduce this technique in the clinical practice for the analysis of larger tissue sections.
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spelling pubmed-97233902022-12-07 Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions Talone, Benedetta Bresci, Arianna Manetti, Francesco Vernuccio, Federico De la Cadena, Alejandro Ceconello, Chiara Schiavone, Maria Lucia Mantero, Stefano Menale, Ciro Vanna, Renzo Cerullo, Giulio Sobacchi, Cristina Polli, Dario Front Bioeng Biotechnol Bioengineering and Biotechnology Bone tissue features a complex microarchitecture and biomolecular composition, which determine biomechanical properties. In addition to state-of-the-art technologies, innovative optical approaches allowing the characterization of the bone in native, label-free conditions can provide new, multi-level insight into this inherently challenging tissue. Here, we exploited multimodal nonlinear optical (NLO) microscopy, including co-registered stimulated Raman scattering, two-photon excited fluorescence, and second-harmonic generation, to image entire vertebrae of murine spine sections. The quantitative nature of these nonlinear interactions allowed us to extract accurate biochemical, morphological, and topological information on the bone tissue and to highlight differences between normal and pathologic samples. Indeed, in a murine model showing bone loss, we observed increased collagen and lipid content as compared to the wild type, along with a decreased craniocaudal alignment of bone collagen fibres. We propose that NLO microscopy can be implemented in standard histopathological analysis of bone in preclinical studies, with the ambitious future perspective to introduce this technique in the clinical practice for the analysis of larger tissue sections. Frontiers Media S.A. 2022-11-22 /pmc/articles/PMC9723390/ /pubmed/36483771 http://dx.doi.org/10.3389/fbioe.2022.1042680 Text en Copyright © 2022 Talone, Bresci, Manetti, Vernuccio, De la Cadena, Ceconello, Schiavone, Mantero, Menale, Vanna, Cerullo, Sobacchi and Polli. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Talone, Benedetta
Bresci, Arianna
Manetti, Francesco
Vernuccio, Federico
De la Cadena, Alejandro
Ceconello, Chiara
Schiavone, Maria Lucia
Mantero, Stefano
Menale, Ciro
Vanna, Renzo
Cerullo, Giulio
Sobacchi, Cristina
Polli, Dario
Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions
title Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions
title_full Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions
title_fullStr Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions
title_full_unstemmed Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions
title_short Label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions
title_sort label-free multimodal nonlinear optical microscopy reveals features of bone composition in pathophysiological conditions
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723390/
https://www.ncbi.nlm.nih.gov/pubmed/36483771
http://dx.doi.org/10.3389/fbioe.2022.1042680
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