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Transcriptional activity differentiates families of Marine Group II Euryarchaeota in the coastal ocean
Marine Group II Euryarchaeota (Candidatus Poseidoniales), abundant but yet-uncultivated members of marine microbial communities, are thought to be (photo)heterotrophs that metabolize dissolved organic matter (DOM), such as lipids and peptides. However, little is known about their transcriptional act...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723583/ https://www.ncbi.nlm.nih.gov/pubmed/37938231 http://dx.doi.org/10.1038/s43705-021-00002-6 |
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author | Damashek, Julian Okotie-Oyekan, Aimee Oyinlade Gifford, Scott Michael Vorobev, Alexey Moran, Mary Ann Hollibaugh, James Timothy |
author_facet | Damashek, Julian Okotie-Oyekan, Aimee Oyinlade Gifford, Scott Michael Vorobev, Alexey Moran, Mary Ann Hollibaugh, James Timothy |
author_sort | Damashek, Julian |
collection | PubMed |
description | Marine Group II Euryarchaeota (Candidatus Poseidoniales), abundant but yet-uncultivated members of marine microbial communities, are thought to be (photo)heterotrophs that metabolize dissolved organic matter (DOM), such as lipids and peptides. However, little is known about their transcriptional activity. We mapped reads from a metatranscriptomic time series collected at Sapelo Island (GA, USA) to metagenome-assembled genomes to determine the diversity of transcriptionally active Ca. Poseidoniales. Summer metatranscriptomes had the highest abundance of Ca. Poseidoniales transcripts, mostly from the O1 and O3 genera within Ca. Thalassarchaeaceae (MGIIb). In contrast, transcripts from fall and winter samples were predominantly from Ca. Poseidoniaceae (MGIIa). Genes encoding proteorhodopsin, membrane-bound pyrophosphatase, peptidase/proteases, and part of the ß-oxidation pathway were highly transcribed across abundant genera. Highly transcribed genes specific to Ca. Thalassarchaeaceae included xanthine/uracil permease and receptors for amino acid transporters. Enrichment of Ca. Thalassarchaeaceae transcript reads related to protein/peptide, nucleic acid, and amino acid transport and metabolism, as well as transcript depletion during dark incubations, provided further evidence of heterotrophic metabolism. Quantitative PCR analysis of South Atlantic Bight samples indicated consistently abundant Ca. Poseidoniales in nearshore and inshore waters. Together, our data suggest that Ca. Thalassarchaeaceae are important photoheterotrophs potentially linking DOM and nitrogen cycling in coastal waters. |
format | Online Article Text |
id | pubmed-9723583 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97235832023-01-04 Transcriptional activity differentiates families of Marine Group II Euryarchaeota in the coastal ocean Damashek, Julian Okotie-Oyekan, Aimee Oyinlade Gifford, Scott Michael Vorobev, Alexey Moran, Mary Ann Hollibaugh, James Timothy ISME Commun Article Marine Group II Euryarchaeota (Candidatus Poseidoniales), abundant but yet-uncultivated members of marine microbial communities, are thought to be (photo)heterotrophs that metabolize dissolved organic matter (DOM), such as lipids and peptides. However, little is known about their transcriptional activity. We mapped reads from a metatranscriptomic time series collected at Sapelo Island (GA, USA) to metagenome-assembled genomes to determine the diversity of transcriptionally active Ca. Poseidoniales. Summer metatranscriptomes had the highest abundance of Ca. Poseidoniales transcripts, mostly from the O1 and O3 genera within Ca. Thalassarchaeaceae (MGIIb). In contrast, transcripts from fall and winter samples were predominantly from Ca. Poseidoniaceae (MGIIa). Genes encoding proteorhodopsin, membrane-bound pyrophosphatase, peptidase/proteases, and part of the ß-oxidation pathway were highly transcribed across abundant genera. Highly transcribed genes specific to Ca. Thalassarchaeaceae included xanthine/uracil permease and receptors for amino acid transporters. Enrichment of Ca. Thalassarchaeaceae transcript reads related to protein/peptide, nucleic acid, and amino acid transport and metabolism, as well as transcript depletion during dark incubations, provided further evidence of heterotrophic metabolism. Quantitative PCR analysis of South Atlantic Bight samples indicated consistently abundant Ca. Poseidoniales in nearshore and inshore waters. Together, our data suggest that Ca. Thalassarchaeaceae are important photoheterotrophs potentially linking DOM and nitrogen cycling in coastal waters. Nature Publishing Group UK 2021-03-22 /pmc/articles/PMC9723583/ /pubmed/37938231 http://dx.doi.org/10.1038/s43705-021-00002-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Damashek, Julian Okotie-Oyekan, Aimee Oyinlade Gifford, Scott Michael Vorobev, Alexey Moran, Mary Ann Hollibaugh, James Timothy Transcriptional activity differentiates families of Marine Group II Euryarchaeota in the coastal ocean |
title | Transcriptional activity differentiates families of Marine Group II Euryarchaeota in the coastal ocean |
title_full | Transcriptional activity differentiates families of Marine Group II Euryarchaeota in the coastal ocean |
title_fullStr | Transcriptional activity differentiates families of Marine Group II Euryarchaeota in the coastal ocean |
title_full_unstemmed | Transcriptional activity differentiates families of Marine Group II Euryarchaeota in the coastal ocean |
title_short | Transcriptional activity differentiates families of Marine Group II Euryarchaeota in the coastal ocean |
title_sort | transcriptional activity differentiates families of marine group ii euryarchaeota in the coastal ocean |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723583/ https://www.ncbi.nlm.nih.gov/pubmed/37938231 http://dx.doi.org/10.1038/s43705-021-00002-6 |
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