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A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells

Telomere elongation is coupled with genome replication, raising the question of the repair of short telomeres in post-mitotic cells. We investigated the fate of a telomere-repeat capped end that mimics a single short telomere in quiescent fission yeast cells. We show that telomerase is able to elong...

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Autores principales: Vaurs, Mélina, Audry, Julien, Runge, Kurt W, Géli, Vincent, Coulon, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723628/
https://www.ncbi.nlm.nih.gov/pubmed/36330920
http://dx.doi.org/10.1093/nar/gkac986
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author Vaurs, Mélina
Audry, Julien
Runge, Kurt W
Géli, Vincent
Coulon, Stéphane
author_facet Vaurs, Mélina
Audry, Julien
Runge, Kurt W
Géli, Vincent
Coulon, Stéphane
author_sort Vaurs, Mélina
collection PubMed
description Telomere elongation is coupled with genome replication, raising the question of the repair of short telomeres in post-mitotic cells. We investigated the fate of a telomere-repeat capped end that mimics a single short telomere in quiescent fission yeast cells. We show that telomerase is able to elongate this single short telomere during quiescence despite the binding of Ku to the proto-telomere. While Taz1 and Rap1 repress telomerase in vegetative cells, both shelterin proteins are required for efficient telomere extension in quiescent cells, underscoring a distinct mode of telomerase control. We further show that Rad3(ATR) and Tel1(ATM) are redundantly required for telomere elongation in quiescence through the phosphorylation of Ccq1 and that Rif1 and its associated-PP1 phosphatases negatively regulate telomerase activity by opposing Ccq1 phosphorylation. The distinct mode of telomerase regulation in quiescent fission yeast cells may be relevant to that in human stem and progenitor cells.
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spelling pubmed-97236282022-12-07 A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells Vaurs, Mélina Audry, Julien Runge, Kurt W Géli, Vincent Coulon, Stéphane Nucleic Acids Res Molecular Biology Telomere elongation is coupled with genome replication, raising the question of the repair of short telomeres in post-mitotic cells. We investigated the fate of a telomere-repeat capped end that mimics a single short telomere in quiescent fission yeast cells. We show that telomerase is able to elongate this single short telomere during quiescence despite the binding of Ku to the proto-telomere. While Taz1 and Rap1 repress telomerase in vegetative cells, both shelterin proteins are required for efficient telomere extension in quiescent cells, underscoring a distinct mode of telomerase control. We further show that Rad3(ATR) and Tel1(ATM) are redundantly required for telomere elongation in quiescence through the phosphorylation of Ccq1 and that Rif1 and its associated-PP1 phosphatases negatively regulate telomerase activity by opposing Ccq1 phosphorylation. The distinct mode of telomerase regulation in quiescent fission yeast cells may be relevant to that in human stem and progenitor cells. Oxford University Press 2022-11-04 /pmc/articles/PMC9723628/ /pubmed/36330920 http://dx.doi.org/10.1093/nar/gkac986 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Molecular Biology
Vaurs, Mélina
Audry, Julien
Runge, Kurt W
Géli, Vincent
Coulon, Stéphane
A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells
title A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells
title_full A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells
title_fullStr A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells
title_full_unstemmed A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells
title_short A proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells
title_sort proto-telomere is elongated by telomerase in a shelterin-dependent manner in quiescent fission yeast cells
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723628/
https://www.ncbi.nlm.nih.gov/pubmed/36330920
http://dx.doi.org/10.1093/nar/gkac986
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