Immunoglobulin somatic hypermutation in a defined biochemical system recapitulates affinity maturation and permits antibody optimization

We describe a purified biochemical system to produce monoclonal antibodies (Abs) in vitro using activation-induced deoxycytidine deaminase (AID) and DNA polymerase η (Polη) to diversify immunoglobulin variable gene (IgV) libraries within a phage display format. AID and Polη function during B-cell af...

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Detalles Bibliográficos
Autores principales: Jeong, Soo Lim, Zhang, Hongyu, Yamaki, Shanni, Yang, Chenyu, McKemy, David D, Lieber, Michael R, Pham, Phuong, Goodman, Myron F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723645/
https://www.ncbi.nlm.nih.gov/pubmed/36321646
http://dx.doi.org/10.1093/nar/gkac995

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723645/
https://www.ncbi.nlm.nih.gov/pubmed/36321646
http://dx.doi.org/10.1093/nar/gkac995

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