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Simultaneous determination of antibiotics residues in edible fish muscle using eco-friendly SPE-UPLC-MS/MS: Occurrence, human dietary exposure and health risk assessment for consumer safety

Occurrence of multi-class antibiotics in edible fish species from the Saudi market was investigated. A fast and sensitive UPLC-MS/MS method for simultaneous determination of selected fluoroquinolones, sulfonamides and macrolides in fish muscle was developed and validated. Sample clean up was perform...

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Detalles Bibliográficos
Autores principales: Shaaban, Heba, Mostafa, Ahmed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723657/
https://www.ncbi.nlm.nih.gov/pubmed/36483750
http://dx.doi.org/10.1016/j.toxrep.2022.11.010
Descripción
Sumario:Occurrence of multi-class antibiotics in edible fish species from the Saudi market was investigated. A fast and sensitive UPLC-MS/MS method for simultaneous determination of selected fluoroquinolones, sulfonamides and macrolides in fish muscle was developed and validated. Sample clean up was performed using solid-phase extraction on Oasis HLB cartridges. The greenness profile of the developed method was evaluated using three assessment tools: analytical Eco-scale assessment method, green analytical procedure index and analytical greenness metric. Detection limits ranged from 0.008 to 0.35 μg/kg. The recovery ranged from 80.1 % to 98.6 % with RSDs ≤ 12.1 %. The mean and maximum concentrations of the detected antibiotics in fish samples ranged between 0.28 and 19.15 and 3.50–112.00 µg/kg, respectively. Human antibiotics (clarithromycin and roxithromycin) were detected in 50 % and 27.5 % of the samples, respectively. The estimated daily intake for the detected antibiotics ranged from 0.10 to 6.61 ng/kg/ BW/day for Saudi adults. The health risk associated with fish consumption was evaluated. The results suggested that fish consumption may not pose a serious risk to consumers in Saudi Arabia. The developed method was fast, sensitive, cost-effective and environmentally friendly.