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Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods

The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics...

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Autores principales: Vezzulli, Luigi, Oliveri, Caterina, Borello, Alessio, Gregory, Lance, Kimirei, Ismael, Brunetta, Martina, Stern, Rowena, Coco, Simona, Longo, Luca, Taviani, Elisa, Santos, Andrès, Martinez-Urtaza, Jaime, Wilson, William H., Colwell, Rita R., Pruzzo, Carla, Plisnier, Pierre-Denis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723668/
https://www.ncbi.nlm.nih.gov/pubmed/37938225
http://dx.doi.org/10.1038/s43705-021-00023-1
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author Vezzulli, Luigi
Oliveri, Caterina
Borello, Alessio
Gregory, Lance
Kimirei, Ismael
Brunetta, Martina
Stern, Rowena
Coco, Simona
Longo, Luca
Taviani, Elisa
Santos, Andrès
Martinez-Urtaza, Jaime
Wilson, William H.
Colwell, Rita R.
Pruzzo, Carla
Plisnier, Pierre-Denis
author_facet Vezzulli, Luigi
Oliveri, Caterina
Borello, Alessio
Gregory, Lance
Kimirei, Ismael
Brunetta, Martina
Stern, Rowena
Coco, Simona
Longo, Luca
Taviani, Elisa
Santos, Andrès
Martinez-Urtaza, Jaime
Wilson, William H.
Colwell, Rita R.
Pruzzo, Carla
Plisnier, Pierre-Denis
author_sort Vezzulli, Luigi
collection PubMed
description The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics, the presence of Vibrio cholerae was investigated in a 96,600 L volume of surface water collected on a 322 nautical mile (596 km) transect in Lake Tanganyika. V. cholerae was detected and identified in a large area of the lake. In contrast, toxigenic strains of V. cholerae O1 or O139 were not detected in plankton samples possibly in relation to environmental conditions of the lake ecosystem, namely very low salinity compared to marine brackish and coastal environments. This represents to our knowledge, the largest environmental study to determine the role of tropical lakes as a reservoir of V. cholerae.
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spelling pubmed-97236682023-01-04 Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods Vezzulli, Luigi Oliveri, Caterina Borello, Alessio Gregory, Lance Kimirei, Ismael Brunetta, Martina Stern, Rowena Coco, Simona Longo, Luca Taviani, Elisa Santos, Andrès Martinez-Urtaza, Jaime Wilson, William H. Colwell, Rita R. Pruzzo, Carla Plisnier, Pierre-Denis ISME Commun Brief Communication The significance of large tropical lakes as environmental reservoirs of Vibrio cholerae in cholera endemic countries has yet to be established. By combining large scale plankton sampling, microbial culture and ultrasensitive molecular methods, namely Droplet Digital PCR (ddPCR) and targeted genomics, the presence of Vibrio cholerae was investigated in a 96,600 L volume of surface water collected on a 322 nautical mile (596 km) transect in Lake Tanganyika. V. cholerae was detected and identified in a large area of the lake. In contrast, toxigenic strains of V. cholerae O1 or O139 were not detected in plankton samples possibly in relation to environmental conditions of the lake ecosystem, namely very low salinity compared to marine brackish and coastal environments. This represents to our knowledge, the largest environmental study to determine the role of tropical lakes as a reservoir of V. cholerae. Nature Publishing Group UK 2021-06-07 /pmc/articles/PMC9723668/ /pubmed/37938225 http://dx.doi.org/10.1038/s43705-021-00023-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Brief Communication
Vezzulli, Luigi
Oliveri, Caterina
Borello, Alessio
Gregory, Lance
Kimirei, Ismael
Brunetta, Martina
Stern, Rowena
Coco, Simona
Longo, Luca
Taviani, Elisa
Santos, Andrès
Martinez-Urtaza, Jaime
Wilson, William H.
Colwell, Rita R.
Pruzzo, Carla
Plisnier, Pierre-Denis
Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods
title Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods
title_full Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods
title_fullStr Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods
title_full_unstemmed Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods
title_short Aquatic reservoir of Vibrio cholerae in an African Great Lake assessed by large scale plankton sampling and ultrasensitive molecular methods
title_sort aquatic reservoir of vibrio cholerae in an african great lake assessed by large scale plankton sampling and ultrasensitive molecular methods
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723668/
https://www.ncbi.nlm.nih.gov/pubmed/37938225
http://dx.doi.org/10.1038/s43705-021-00023-1
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