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Viral diversity is linked to bacterial community composition in alpine stream biofilms
Biofilms play pivotal roles in fluvial ecosystems, yet virtually nothing is known about viruses in these communities. Leveraging an optimized sample-to-sequence pipeline, we studied the spatiotemporal turnover of dsDNA viruses associated with stream biofilms and found an astounding diversity to be s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723757/ https://www.ncbi.nlm.nih.gov/pubmed/37938299 http://dx.doi.org/10.1038/s43705-022-00112-9 |
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author | Bekliz, Meriem Pramateftaki, Paraskevi Battin, Tom Jan Peter, Hannes |
author_facet | Bekliz, Meriem Pramateftaki, Paraskevi Battin, Tom Jan Peter, Hannes |
author_sort | Bekliz, Meriem |
collection | PubMed |
description | Biofilms play pivotal roles in fluvial ecosystems, yet virtually nothing is known about viruses in these communities. Leveraging an optimized sample-to-sequence pipeline, we studied the spatiotemporal turnover of dsDNA viruses associated with stream biofilms and found an astounding diversity to be structured by seasons and along the longitudinal gradient in the stream. While some vOTUs were region- or season-specific, we also identified a large group of permanent biofilm phages, taxonomically dominated by Myoviridae. Comparison of the observed viral distribution with predictions based on neutral community assembly indicated that chance and dispersal may be important for structuring stream biofilm viral communities. Deviation from neutral model predictions suggests that certain phages distribute efficiently across distant locations within the stream network. This dispersal capacity appears to be linked to EPS depolymerases that enable phages to efficiently overcome the biofilm barrier. Other phages, particularly vOTUs classified as Siphoviridae, appear locally overrepresented and to rely on a lysogenic life cycle, potentially to exploit the spatial distribution of bacterial populations in stream biofilms. Overall, biofilm viral and bacterial community turnover were significantly coupled. Yet, viral communities were linked to the presence of the most abundant bacterial community members. With this work, we provide a foundational ecological perspective on factors that structure viral diversity in stream biofilms and identify potentially important viral traits related to the biofilm mode of life. |
format | Online Article Text |
id | pubmed-9723757 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97237572023-01-04 Viral diversity is linked to bacterial community composition in alpine stream biofilms Bekliz, Meriem Pramateftaki, Paraskevi Battin, Tom Jan Peter, Hannes ISME Commun Article Biofilms play pivotal roles in fluvial ecosystems, yet virtually nothing is known about viruses in these communities. Leveraging an optimized sample-to-sequence pipeline, we studied the spatiotemporal turnover of dsDNA viruses associated with stream biofilms and found an astounding diversity to be structured by seasons and along the longitudinal gradient in the stream. While some vOTUs were region- or season-specific, we also identified a large group of permanent biofilm phages, taxonomically dominated by Myoviridae. Comparison of the observed viral distribution with predictions based on neutral community assembly indicated that chance and dispersal may be important for structuring stream biofilm viral communities. Deviation from neutral model predictions suggests that certain phages distribute efficiently across distant locations within the stream network. This dispersal capacity appears to be linked to EPS depolymerases that enable phages to efficiently overcome the biofilm barrier. Other phages, particularly vOTUs classified as Siphoviridae, appear locally overrepresented and to rely on a lysogenic life cycle, potentially to exploit the spatial distribution of bacterial populations in stream biofilms. Overall, biofilm viral and bacterial community turnover were significantly coupled. Yet, viral communities were linked to the presence of the most abundant bacterial community members. With this work, we provide a foundational ecological perspective on factors that structure viral diversity in stream biofilms and identify potentially important viral traits related to the biofilm mode of life. Nature Publishing Group UK 2022-03-30 /pmc/articles/PMC9723757/ /pubmed/37938299 http://dx.doi.org/10.1038/s43705-022-00112-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Bekliz, Meriem Pramateftaki, Paraskevi Battin, Tom Jan Peter, Hannes Viral diversity is linked to bacterial community composition in alpine stream biofilms |
title | Viral diversity is linked to bacterial community composition in alpine stream biofilms |
title_full | Viral diversity is linked to bacterial community composition in alpine stream biofilms |
title_fullStr | Viral diversity is linked to bacterial community composition in alpine stream biofilms |
title_full_unstemmed | Viral diversity is linked to bacterial community composition in alpine stream biofilms |
title_short | Viral diversity is linked to bacterial community composition in alpine stream biofilms |
title_sort | viral diversity is linked to bacterial community composition in alpine stream biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9723757/ https://www.ncbi.nlm.nih.gov/pubmed/37938299 http://dx.doi.org/10.1038/s43705-022-00112-9 |
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