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Sourcing cells for in vitro models of human vascular barriers of inflammation
The vascular system plays a critical role in the progression and resolution of inflammation. The contributions of the vascular endothelium to these processes, however, vary with tissue and disease state. Recently, tissue chip models have emerged as promising tools to understand human disease and for...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724237/ https://www.ncbi.nlm.nih.gov/pubmed/36483299 http://dx.doi.org/10.3389/fmedt.2022.979768 |
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author | McCloskey, Molly C. Zhang, Victor Z. Ahmad, S. Danial Walker, Samuel Romanick, Samantha S. Awad, Hani A. McGrath, James L. |
author_facet | McCloskey, Molly C. Zhang, Victor Z. Ahmad, S. Danial Walker, Samuel Romanick, Samantha S. Awad, Hani A. McGrath, James L. |
author_sort | McCloskey, Molly C. |
collection | PubMed |
description | The vascular system plays a critical role in the progression and resolution of inflammation. The contributions of the vascular endothelium to these processes, however, vary with tissue and disease state. Recently, tissue chip models have emerged as promising tools to understand human disease and for the development of personalized medicine approaches. Inclusion of a vascular component within these platforms is critical for properly evaluating most diseases, but many models to date use “generic” endothelial cells, which can preclude the identification of biomedically meaningful pathways and mechanisms. As the knowledge of vascular heterogeneity and immune cell trafficking throughout the body advances, tissue chip models should also advance to incorporate tissue-specific cells where possible. Here, we discuss the known heterogeneity of leukocyte trafficking in vascular beds of some commonly modeled tissues. We comment on the availability of different tissue-specific cell sources for endothelial cells and pericytes, with a focus on stem cell sources for the full realization of personalized medicine. We discuss sources available for the immune cells needed to model inflammatory processes and the findings of tissue chip models that have used the cells to studying transmigration. |
format | Online Article Text |
id | pubmed-9724237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97242372022-12-07 Sourcing cells for in vitro models of human vascular barriers of inflammation McCloskey, Molly C. Zhang, Victor Z. Ahmad, S. Danial Walker, Samuel Romanick, Samantha S. Awad, Hani A. McGrath, James L. Front Med Technol Medical Technology The vascular system plays a critical role in the progression and resolution of inflammation. The contributions of the vascular endothelium to these processes, however, vary with tissue and disease state. Recently, tissue chip models have emerged as promising tools to understand human disease and for the development of personalized medicine approaches. Inclusion of a vascular component within these platforms is critical for properly evaluating most diseases, but many models to date use “generic” endothelial cells, which can preclude the identification of biomedically meaningful pathways and mechanisms. As the knowledge of vascular heterogeneity and immune cell trafficking throughout the body advances, tissue chip models should also advance to incorporate tissue-specific cells where possible. Here, we discuss the known heterogeneity of leukocyte trafficking in vascular beds of some commonly modeled tissues. We comment on the availability of different tissue-specific cell sources for endothelial cells and pericytes, with a focus on stem cell sources for the full realization of personalized medicine. We discuss sources available for the immune cells needed to model inflammatory processes and the findings of tissue chip models that have used the cells to studying transmigration. Frontiers Media S.A. 2022-10-21 /pmc/articles/PMC9724237/ /pubmed/36483299 http://dx.doi.org/10.3389/fmedt.2022.979768 Text en © 2022 McCloskey, Zhang, Ahmad, Walker, Romanick, Awad and McGrath. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Medical Technology McCloskey, Molly C. Zhang, Victor Z. Ahmad, S. Danial Walker, Samuel Romanick, Samantha S. Awad, Hani A. McGrath, James L. Sourcing cells for in vitro models of human vascular barriers of inflammation |
title | Sourcing cells for in vitro models of human vascular barriers of inflammation |
title_full | Sourcing cells for in vitro models of human vascular barriers of inflammation |
title_fullStr | Sourcing cells for in vitro models of human vascular barriers of inflammation |
title_full_unstemmed | Sourcing cells for in vitro models of human vascular barriers of inflammation |
title_short | Sourcing cells for in vitro models of human vascular barriers of inflammation |
title_sort | sourcing cells for in vitro models of human vascular barriers of inflammation |
topic | Medical Technology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724237/ https://www.ncbi.nlm.nih.gov/pubmed/36483299 http://dx.doi.org/10.3389/fmedt.2022.979768 |
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