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Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B

Background & Aims: Accurately identifying liver necroinflammation was essential for the timely implementation of antiviral therapy in chronic hepatitis B(CHB) patients. The sphingolipids were involved in various chronic inflammatory processes. This study aimed to evaluate the association between...

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Autores principales: Ren, Yan, Zhao, Jing, Xu, Manman, Wang, Yang, Bai, Li, Jiang, Yingying, Liu, Shuang, Chen, Yu, Duan, Zhongping, Zheng, Sujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724247/
https://www.ncbi.nlm.nih.gov/pubmed/36483591
http://dx.doi.org/10.7150/ijms.75820
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author Ren, Yan
Zhao, Jing
Xu, Manman
Wang, Yang
Bai, Li
Jiang, Yingying
Liu, Shuang
Chen, Yu
Duan, Zhongping
Zheng, Sujun
author_facet Ren, Yan
Zhao, Jing
Xu, Manman
Wang, Yang
Bai, Li
Jiang, Yingying
Liu, Shuang
Chen, Yu
Duan, Zhongping
Zheng, Sujun
author_sort Ren, Yan
collection PubMed
description Background & Aims: Accurately identifying liver necroinflammation was essential for the timely implementation of antiviral therapy in chronic hepatitis B(CHB) patients. The sphingolipids were involved in various chronic inflammatory processes. This study aimed to evaluate the association between serum sphingolipids and liver necroinflammation in CHB patients. Methods: The study prospectively enrolled patients with a diagnosis of chronic hepatitis B who were subsequently treated with nucleos(t)ide analogs (NAs). Liver biopsy was performed at baseline and 5-year follow-up, and serum sphingolipid levels were measured by ultra-high-performance liquid chromatography tandem mass spectrometry. Results: A total of 70 CHB patients were enrolled with baseline liver necroinflammation of 27(38.6%) G1, 23(32.9%) G2, and 20(28.6%) G ≥ 3, respectively. A total of 126 liver biopsies were performed on the study population over a 5-year period, of which 80 (63.5%) G<2 and 46 (36.5%) G≥2. Serum ALT, ALP, SM d16:0/16:1, SM d16:0/17:1, SM d18:0/17:0 and Cer d18:2/22:0 showed significant differences between two groups (P<0.01). Multivariate analysis showed that serum ALT (OR 1.006, 95% CI: 1.000-1.011), SM d16:0/16:1 (OR 1.552, 95% CI: 1.150-2.093), Cer d18:2/22:0 (OR 0.003, 95% CI: 0.000-0.173) were associated with G ≥ 2. In the subgroup of patients with normal serum ALT, serum Cer d18:2/22:0 was lower in patients with G ≥ 2 than that with G < 2. After 5 years, alleviated inflammation was accompanied by decreased serum SM d16:0/16:1 and increased serum Cer d18:2/22:0 in patients with baseline G ≥ 2. Conclusions: Lower serum Cer d18:2/22:0 could reflect hepatic necroinflammation (G ≥ 2) in CHB patients including those with normal serum ALT, and its elevation predicts the inflammation improvement after NAs treatment.
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spelling pubmed-97242472022-12-07 Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B Ren, Yan Zhao, Jing Xu, Manman Wang, Yang Bai, Li Jiang, Yingying Liu, Shuang Chen, Yu Duan, Zhongping Zheng, Sujun Int J Med Sci Research Paper Background & Aims: Accurately identifying liver necroinflammation was essential for the timely implementation of antiviral therapy in chronic hepatitis B(CHB) patients. The sphingolipids were involved in various chronic inflammatory processes. This study aimed to evaluate the association between serum sphingolipids and liver necroinflammation in CHB patients. Methods: The study prospectively enrolled patients with a diagnosis of chronic hepatitis B who were subsequently treated with nucleos(t)ide analogs (NAs). Liver biopsy was performed at baseline and 5-year follow-up, and serum sphingolipid levels were measured by ultra-high-performance liquid chromatography tandem mass spectrometry. Results: A total of 70 CHB patients were enrolled with baseline liver necroinflammation of 27(38.6%) G1, 23(32.9%) G2, and 20(28.6%) G ≥ 3, respectively. A total of 126 liver biopsies were performed on the study population over a 5-year period, of which 80 (63.5%) G<2 and 46 (36.5%) G≥2. Serum ALT, ALP, SM d16:0/16:1, SM d16:0/17:1, SM d18:0/17:0 and Cer d18:2/22:0 showed significant differences between two groups (P<0.01). Multivariate analysis showed that serum ALT (OR 1.006, 95% CI: 1.000-1.011), SM d16:0/16:1 (OR 1.552, 95% CI: 1.150-2.093), Cer d18:2/22:0 (OR 0.003, 95% CI: 0.000-0.173) were associated with G ≥ 2. In the subgroup of patients with normal serum ALT, serum Cer d18:2/22:0 was lower in patients with G ≥ 2 than that with G < 2. After 5 years, alleviated inflammation was accompanied by decreased serum SM d16:0/16:1 and increased serum Cer d18:2/22:0 in patients with baseline G ≥ 2. Conclusions: Lower serum Cer d18:2/22:0 could reflect hepatic necroinflammation (G ≥ 2) in CHB patients including those with normal serum ALT, and its elevation predicts the inflammation improvement after NAs treatment. Ivyspring International Publisher 2022-11-21 /pmc/articles/PMC9724247/ /pubmed/36483591 http://dx.doi.org/10.7150/ijms.75820 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Ren, Yan
Zhao, Jing
Xu, Manman
Wang, Yang
Bai, Li
Jiang, Yingying
Liu, Shuang
Chen, Yu
Duan, Zhongping
Zheng, Sujun
Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B
title Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B
title_full Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B
title_fullStr Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B
title_full_unstemmed Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B
title_short Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B
title_sort association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis b
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724247/
https://www.ncbi.nlm.nih.gov/pubmed/36483591
http://dx.doi.org/10.7150/ijms.75820
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