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Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B
Background & Aims: Accurately identifying liver necroinflammation was essential for the timely implementation of antiviral therapy in chronic hepatitis B(CHB) patients. The sphingolipids were involved in various chronic inflammatory processes. This study aimed to evaluate the association between...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724247/ https://www.ncbi.nlm.nih.gov/pubmed/36483591 http://dx.doi.org/10.7150/ijms.75820 |
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author | Ren, Yan Zhao, Jing Xu, Manman Wang, Yang Bai, Li Jiang, Yingying Liu, Shuang Chen, Yu Duan, Zhongping Zheng, Sujun |
author_facet | Ren, Yan Zhao, Jing Xu, Manman Wang, Yang Bai, Li Jiang, Yingying Liu, Shuang Chen, Yu Duan, Zhongping Zheng, Sujun |
author_sort | Ren, Yan |
collection | PubMed |
description | Background & Aims: Accurately identifying liver necroinflammation was essential for the timely implementation of antiviral therapy in chronic hepatitis B(CHB) patients. The sphingolipids were involved in various chronic inflammatory processes. This study aimed to evaluate the association between serum sphingolipids and liver necroinflammation in CHB patients. Methods: The study prospectively enrolled patients with a diagnosis of chronic hepatitis B who were subsequently treated with nucleos(t)ide analogs (NAs). Liver biopsy was performed at baseline and 5-year follow-up, and serum sphingolipid levels were measured by ultra-high-performance liquid chromatography tandem mass spectrometry. Results: A total of 70 CHB patients were enrolled with baseline liver necroinflammation of 27(38.6%) G1, 23(32.9%) G2, and 20(28.6%) G ≥ 3, respectively. A total of 126 liver biopsies were performed on the study population over a 5-year period, of which 80 (63.5%) G<2 and 46 (36.5%) G≥2. Serum ALT, ALP, SM d16:0/16:1, SM d16:0/17:1, SM d18:0/17:0 and Cer d18:2/22:0 showed significant differences between two groups (P<0.01). Multivariate analysis showed that serum ALT (OR 1.006, 95% CI: 1.000-1.011), SM d16:0/16:1 (OR 1.552, 95% CI: 1.150-2.093), Cer d18:2/22:0 (OR 0.003, 95% CI: 0.000-0.173) were associated with G ≥ 2. In the subgroup of patients with normal serum ALT, serum Cer d18:2/22:0 was lower in patients with G ≥ 2 than that with G < 2. After 5 years, alleviated inflammation was accompanied by decreased serum SM d16:0/16:1 and increased serum Cer d18:2/22:0 in patients with baseline G ≥ 2. Conclusions: Lower serum Cer d18:2/22:0 could reflect hepatic necroinflammation (G ≥ 2) in CHB patients including those with normal serum ALT, and its elevation predicts the inflammation improvement after NAs treatment. |
format | Online Article Text |
id | pubmed-9724247 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-97242472022-12-07 Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B Ren, Yan Zhao, Jing Xu, Manman Wang, Yang Bai, Li Jiang, Yingying Liu, Shuang Chen, Yu Duan, Zhongping Zheng, Sujun Int J Med Sci Research Paper Background & Aims: Accurately identifying liver necroinflammation was essential for the timely implementation of antiviral therapy in chronic hepatitis B(CHB) patients. The sphingolipids were involved in various chronic inflammatory processes. This study aimed to evaluate the association between serum sphingolipids and liver necroinflammation in CHB patients. Methods: The study prospectively enrolled patients with a diagnosis of chronic hepatitis B who were subsequently treated with nucleos(t)ide analogs (NAs). Liver biopsy was performed at baseline and 5-year follow-up, and serum sphingolipid levels were measured by ultra-high-performance liquid chromatography tandem mass spectrometry. Results: A total of 70 CHB patients were enrolled with baseline liver necroinflammation of 27(38.6%) G1, 23(32.9%) G2, and 20(28.6%) G ≥ 3, respectively. A total of 126 liver biopsies were performed on the study population over a 5-year period, of which 80 (63.5%) G<2 and 46 (36.5%) G≥2. Serum ALT, ALP, SM d16:0/16:1, SM d16:0/17:1, SM d18:0/17:0 and Cer d18:2/22:0 showed significant differences between two groups (P<0.01). Multivariate analysis showed that serum ALT (OR 1.006, 95% CI: 1.000-1.011), SM d16:0/16:1 (OR 1.552, 95% CI: 1.150-2.093), Cer d18:2/22:0 (OR 0.003, 95% CI: 0.000-0.173) were associated with G ≥ 2. In the subgroup of patients with normal serum ALT, serum Cer d18:2/22:0 was lower in patients with G ≥ 2 than that with G < 2. After 5 years, alleviated inflammation was accompanied by decreased serum SM d16:0/16:1 and increased serum Cer d18:2/22:0 in patients with baseline G ≥ 2. Conclusions: Lower serum Cer d18:2/22:0 could reflect hepatic necroinflammation (G ≥ 2) in CHB patients including those with normal serum ALT, and its elevation predicts the inflammation improvement after NAs treatment. Ivyspring International Publisher 2022-11-21 /pmc/articles/PMC9724247/ /pubmed/36483591 http://dx.doi.org/10.7150/ijms.75820 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Ren, Yan Zhao, Jing Xu, Manman Wang, Yang Bai, Li Jiang, Yingying Liu, Shuang Chen, Yu Duan, Zhongping Zheng, Sujun Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B |
title | Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B |
title_full | Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B |
title_fullStr | Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B |
title_full_unstemmed | Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B |
title_short | Association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis B |
title_sort | association between serum sphingolipids and necroinflammation of liver tissue pathology in chronic hepatitis b |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724247/ https://www.ncbi.nlm.nih.gov/pubmed/36483591 http://dx.doi.org/10.7150/ijms.75820 |
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