Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene

BACKGROUND: Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent vaccines based on it, the reverse genetics system f...

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Autores principales: He, Lei, Wang, Hairong, Yu, Zuhua, Liao, Chengshui, Ding, Ke, Zhang, Cai, Yu, Chuan, Zhang, Chunjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724440/
https://www.ncbi.nlm.nih.gov/pubmed/36471312
http://dx.doi.org/10.1186/s12896-022-00763-5
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author He, Lei
Wang, Hairong
Yu, Zuhua
Liao, Chengshui
Ding, Ke
Zhang, Cai
Yu, Chuan
Zhang, Chunjie
author_facet He, Lei
Wang, Hairong
Yu, Zuhua
Liao, Chengshui
Ding, Ke
Zhang, Cai
Yu, Chuan
Zhang, Chunjie
author_sort He, Lei
collection PubMed
description BACKGROUND: Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent vaccines based on it, the reverse genetics system for NDV ZM10 is an indispensable platform. RESULTS: A full-length cDNA clone of NDV ZM10 and three supporting plasmids were constructed using the ligation-independent cloning method. Recombinant NDV rZM10 was successfully rescued after these plasmids were co-transfected into BHK-21 cells. Besides, the recombinant virus rZM10-RFP encoding the red fluorescent protein was generated by inserting the RFP gene into the full-length clone of NDV between the P and M genes. These rescued viruses were genetically and biologically identical to the parental strain and showed similar growth kinetics. CONCLUSION: The recovery system of NDV ZM10 strain was established, and can be used as a foundation for research on the enterotropic mechanism and development of multivalent vaccines against viral diseases of livestock and poultry. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12896-022-00763-5.
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spelling pubmed-97244402022-12-07 Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene He, Lei Wang, Hairong Yu, Zuhua Liao, Chengshui Ding, Ke Zhang, Cai Yu, Chuan Zhang, Chunjie BMC Biotechnol Research BACKGROUND: Newcastle disease virus (NDV) strain ZM10, a typical enterotropic avirulent vaccine strain, has been widely used in China for chickens against Newcastle disease. To elucidate its enterotropic mechanism and develop recombiant multivalent vaccines based on it, the reverse genetics system for NDV ZM10 is an indispensable platform. RESULTS: A full-length cDNA clone of NDV ZM10 and three supporting plasmids were constructed using the ligation-independent cloning method. Recombinant NDV rZM10 was successfully rescued after these plasmids were co-transfected into BHK-21 cells. Besides, the recombinant virus rZM10-RFP encoding the red fluorescent protein was generated by inserting the RFP gene into the full-length clone of NDV between the P and M genes. These rescued viruses were genetically and biologically identical to the parental strain and showed similar growth kinetics. CONCLUSION: The recovery system of NDV ZM10 strain was established, and can be used as a foundation for research on the enterotropic mechanism and development of multivalent vaccines against viral diseases of livestock and poultry. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12896-022-00763-5. BioMed Central 2022-12-06 /pmc/articles/PMC9724440/ /pubmed/36471312 http://dx.doi.org/10.1186/s12896-022-00763-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
He, Lei
Wang, Hairong
Yu, Zuhua
Liao, Chengshui
Ding, Ke
Zhang, Cai
Yu, Chuan
Zhang, Chunjie
Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene
title Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene
title_full Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene
title_fullStr Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene
title_full_unstemmed Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene
title_short Rescue of an enterotropic Newcastle disease virus strain ZM10 from cloned cDNA and stable expressing an inserted foreign gene
title_sort rescue of an enterotropic newcastle disease virus strain zm10 from cloned cdna and stable expressing an inserted foreign gene
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724440/
https://www.ncbi.nlm.nih.gov/pubmed/36471312
http://dx.doi.org/10.1186/s12896-022-00763-5
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