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Infection of SARS-CoV-2 causes severe pathological changes in mouse testis

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has affected more than 600 million people worldwide. Several organs including lung, intestine, and brain are infected by SARS-CoV-2. It has been reported that SARS-CoV-2 receptor angiotensin-...

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Autores principales: Chen, Min, Li, Shihua, Liu, Shujun, Zhang, Yuhang, Cui, Xiuhong, Lv, Limin, Liu, Bowen, Zheng, Aihua, Wang, Qihui, Duo, Shuguang, Gao, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724560/
https://www.ncbi.nlm.nih.gov/pubmed/36494057
http://dx.doi.org/10.1016/j.jgg.2022.11.011
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author Chen, Min
Li, Shihua
Liu, Shujun
Zhang, Yuhang
Cui, Xiuhong
Lv, Limin
Liu, Bowen
Zheng, Aihua
Wang, Qihui
Duo, Shuguang
Gao, Fei
author_facet Chen, Min
Li, Shihua
Liu, Shujun
Zhang, Yuhang
Cui, Xiuhong
Lv, Limin
Liu, Bowen
Zheng, Aihua
Wang, Qihui
Duo, Shuguang
Gao, Fei
author_sort Chen, Min
collection PubMed
description Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has affected more than 600 million people worldwide. Several organs including lung, intestine, and brain are infected by SARS-CoV-2. It has been reported that SARS-CoV-2 receptor angiotensin-converting enzyme-2 (ACE2) is expressed in human testis. However, whether testis is also affected by SARS-CoV-2 is still unclear. In this study, we generate a human ACE2 (hACE2) transgenic mouse model in which the expression of hACE2 gene is regulated by hACE2 promoter. Sertoli and Leydig cells from hACE2 transgenic mice can be infected by SARS-CoV-2 pseudovirus in vitro, and severe pathological changes are observed after injecting the SARS-CoV-2 pseudovirus into the seminiferous tubules. Further studies reveal that Sertoli and Leydig cells from hACE2 transgenic mice are also infected by authentic SARS-CoV-2 virus in vitro. After testis interstitium injection, authentic SARS-CoV-2 viruses are first disseminated to the interstitial cells, and then detected inside the seminiferous tubules which in turn cause germ cell loss and disruption of seminiferous tubules. Our study demonstrates that testis is most likely a target of SARS-CoV-2 virus. Attention should be paid to the reproductive function in SARS-CoV-2 patients.
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spelling pubmed-97245602022-12-06 Infection of SARS-CoV-2 causes severe pathological changes in mouse testis Chen, Min Li, Shihua Liu, Shujun Zhang, Yuhang Cui, Xiuhong Lv, Limin Liu, Bowen Zheng, Aihua Wang, Qihui Duo, Shuguang Gao, Fei J Genet Genomics Original Research Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has affected more than 600 million people worldwide. Several organs including lung, intestine, and brain are infected by SARS-CoV-2. It has been reported that SARS-CoV-2 receptor angiotensin-converting enzyme-2 (ACE2) is expressed in human testis. However, whether testis is also affected by SARS-CoV-2 is still unclear. In this study, we generate a human ACE2 (hACE2) transgenic mouse model in which the expression of hACE2 gene is regulated by hACE2 promoter. Sertoli and Leydig cells from hACE2 transgenic mice can be infected by SARS-CoV-2 pseudovirus in vitro, and severe pathological changes are observed after injecting the SARS-CoV-2 pseudovirus into the seminiferous tubules. Further studies reveal that Sertoli and Leydig cells from hACE2 transgenic mice are also infected by authentic SARS-CoV-2 virus in vitro. After testis interstitium injection, authentic SARS-CoV-2 viruses are first disseminated to the interstitial cells, and then detected inside the seminiferous tubules which in turn cause germ cell loss and disruption of seminiferous tubules. Our study demonstrates that testis is most likely a target of SARS-CoV-2 virus. Attention should be paid to the reproductive function in SARS-CoV-2 patients. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. 2023-02 2022-12-06 /pmc/articles/PMC9724560/ /pubmed/36494057 http://dx.doi.org/10.1016/j.jgg.2022.11.011 Text en © 2023 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Original Research
Chen, Min
Li, Shihua
Liu, Shujun
Zhang, Yuhang
Cui, Xiuhong
Lv, Limin
Liu, Bowen
Zheng, Aihua
Wang, Qihui
Duo, Shuguang
Gao, Fei
Infection of SARS-CoV-2 causes severe pathological changes in mouse testis
title Infection of SARS-CoV-2 causes severe pathological changes in mouse testis
title_full Infection of SARS-CoV-2 causes severe pathological changes in mouse testis
title_fullStr Infection of SARS-CoV-2 causes severe pathological changes in mouse testis
title_full_unstemmed Infection of SARS-CoV-2 causes severe pathological changes in mouse testis
title_short Infection of SARS-CoV-2 causes severe pathological changes in mouse testis
title_sort infection of sars-cov-2 causes severe pathological changes in mouse testis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724560/
https://www.ncbi.nlm.nih.gov/pubmed/36494057
http://dx.doi.org/10.1016/j.jgg.2022.11.011
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