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Infection of SARS-CoV-2 causes severe pathological changes in mouse testis
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has affected more than 600 million people worldwide. Several organs including lung, intestine, and brain are infected by SARS-CoV-2. It has been reported that SARS-CoV-2 receptor angiotensin-...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724560/ https://www.ncbi.nlm.nih.gov/pubmed/36494057 http://dx.doi.org/10.1016/j.jgg.2022.11.011 |
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author | Chen, Min Li, Shihua Liu, Shujun Zhang, Yuhang Cui, Xiuhong Lv, Limin Liu, Bowen Zheng, Aihua Wang, Qihui Duo, Shuguang Gao, Fei |
author_facet | Chen, Min Li, Shihua Liu, Shujun Zhang, Yuhang Cui, Xiuhong Lv, Limin Liu, Bowen Zheng, Aihua Wang, Qihui Duo, Shuguang Gao, Fei |
author_sort | Chen, Min |
collection | PubMed |
description | Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has affected more than 600 million people worldwide. Several organs including lung, intestine, and brain are infected by SARS-CoV-2. It has been reported that SARS-CoV-2 receptor angiotensin-converting enzyme-2 (ACE2) is expressed in human testis. However, whether testis is also affected by SARS-CoV-2 is still unclear. In this study, we generate a human ACE2 (hACE2) transgenic mouse model in which the expression of hACE2 gene is regulated by hACE2 promoter. Sertoli and Leydig cells from hACE2 transgenic mice can be infected by SARS-CoV-2 pseudovirus in vitro, and severe pathological changes are observed after injecting the SARS-CoV-2 pseudovirus into the seminiferous tubules. Further studies reveal that Sertoli and Leydig cells from hACE2 transgenic mice are also infected by authentic SARS-CoV-2 virus in vitro. After testis interstitium injection, authentic SARS-CoV-2 viruses are first disseminated to the interstitial cells, and then detected inside the seminiferous tubules which in turn cause germ cell loss and disruption of seminiferous tubules. Our study demonstrates that testis is most likely a target of SARS-CoV-2 virus. Attention should be paid to the reproductive function in SARS-CoV-2 patients. |
format | Online Article Text |
id | pubmed-9724560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97245602022-12-06 Infection of SARS-CoV-2 causes severe pathological changes in mouse testis Chen, Min Li, Shihua Liu, Shujun Zhang, Yuhang Cui, Xiuhong Lv, Limin Liu, Bowen Zheng, Aihua Wang, Qihui Duo, Shuguang Gao, Fei J Genet Genomics Original Research Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has affected more than 600 million people worldwide. Several organs including lung, intestine, and brain are infected by SARS-CoV-2. It has been reported that SARS-CoV-2 receptor angiotensin-converting enzyme-2 (ACE2) is expressed in human testis. However, whether testis is also affected by SARS-CoV-2 is still unclear. In this study, we generate a human ACE2 (hACE2) transgenic mouse model in which the expression of hACE2 gene is regulated by hACE2 promoter. Sertoli and Leydig cells from hACE2 transgenic mice can be infected by SARS-CoV-2 pseudovirus in vitro, and severe pathological changes are observed after injecting the SARS-CoV-2 pseudovirus into the seminiferous tubules. Further studies reveal that Sertoli and Leydig cells from hACE2 transgenic mice are also infected by authentic SARS-CoV-2 virus in vitro. After testis interstitium injection, authentic SARS-CoV-2 viruses are first disseminated to the interstitial cells, and then detected inside the seminiferous tubules which in turn cause germ cell loss and disruption of seminiferous tubules. Our study demonstrates that testis is most likely a target of SARS-CoV-2 virus. Attention should be paid to the reproductive function in SARS-CoV-2 patients. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. 2023-02 2022-12-06 /pmc/articles/PMC9724560/ /pubmed/36494057 http://dx.doi.org/10.1016/j.jgg.2022.11.011 Text en © 2023 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Original Research Chen, Min Li, Shihua Liu, Shujun Zhang, Yuhang Cui, Xiuhong Lv, Limin Liu, Bowen Zheng, Aihua Wang, Qihui Duo, Shuguang Gao, Fei Infection of SARS-CoV-2 causes severe pathological changes in mouse testis |
title | Infection of SARS-CoV-2 causes severe pathological changes in mouse testis |
title_full | Infection of SARS-CoV-2 causes severe pathological changes in mouse testis |
title_fullStr | Infection of SARS-CoV-2 causes severe pathological changes in mouse testis |
title_full_unstemmed | Infection of SARS-CoV-2 causes severe pathological changes in mouse testis |
title_short | Infection of SARS-CoV-2 causes severe pathological changes in mouse testis |
title_sort | infection of sars-cov-2 causes severe pathological changes in mouse testis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724560/ https://www.ncbi.nlm.nih.gov/pubmed/36494057 http://dx.doi.org/10.1016/j.jgg.2022.11.011 |
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