Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus

• Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection. • The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA. • No cross-reaction was found in 14 rash and fever-associated viruses. • The two RPA methods are simple and rapid, maki...

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Detalles Bibliográficos
Autores principales: Li, Yuchang, Gao, Yanhong, Tang, Ying, Li, Jing, Zhang, Sen, Jiang, Tao, Kang, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724568/
https://www.ncbi.nlm.nih.gov/pubmed/36494078
http://dx.doi.org/10.1016/j.virs.2022.12.001
Descripción
Sumario:• Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection. • The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA. • No cross-reaction was found in 14 rash and fever-associated viruses. • The two RPA methods are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.

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