Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus

• Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection. • The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA. • No cross-reaction was found in 14 rash and fever-associated viruses. • The two RPA methods are simple and rapid, maki...

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Autores principales: Li, Yuchang, Gao, Yanhong, Tang, Ying, Li, Jing, Zhang, Sen, Jiang, Tao, Kang, Xiaoping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724568/
https://www.ncbi.nlm.nih.gov/pubmed/36494078
http://dx.doi.org/10.1016/j.virs.2022.12.001
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author Li, Yuchang
Gao, Yanhong
Tang, Ying
Li, Jing
Zhang, Sen
Jiang, Tao
Kang, Xiaoping
author_facet Li, Yuchang
Gao, Yanhong
Tang, Ying
Li, Jing
Zhang, Sen
Jiang, Tao
Kang, Xiaoping
author_sort Li, Yuchang
collection PubMed
description • Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection. • The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA. • No cross-reaction was found in 14 rash and fever-associated viruses. • The two RPA methods are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.
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spelling pubmed-97245682022-12-07 Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus Li, Yuchang Gao, Yanhong Tang, Ying Li, Jing Zhang, Sen Jiang, Tao Kang, Xiaoping Virol Sin Letter • Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection. • The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA. • No cross-reaction was found in 14 rash and fever-associated viruses. • The two RPA methods are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-12-06 /pmc/articles/PMC9724568/ /pubmed/36494078 http://dx.doi.org/10.1016/j.virs.2022.12.001 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Letter
Li, Yuchang
Gao, Yanhong
Tang, Ying
Li, Jing
Zhang, Sen
Jiang, Tao
Kang, Xiaoping
Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus
title Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus
title_full Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus
title_fullStr Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus
title_full_unstemmed Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus
title_short Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus
title_sort development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724568/
https://www.ncbi.nlm.nih.gov/pubmed/36494078
http://dx.doi.org/10.1016/j.virs.2022.12.001
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