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Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein

In this work, we report an impedimetric system for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein. The sensing platform is based on recombinant Spike protein (SCoV2-rS) immobilized on the phytic acid doped polyaniline films (PANI-PA). T...

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Detalles Bibliográficos
Autores principales: Drobysh, Maryia, Ramanavicius, Arunas, Baradoke, Ausra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726207/
https://www.ncbi.nlm.nih.gov/pubmed/36493838
http://dx.doi.org/10.1016/j.scitotenv.2022.160700
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author Drobysh, Maryia
Ramanavicius, Arunas
Baradoke, Ausra
author_facet Drobysh, Maryia
Ramanavicius, Arunas
Baradoke, Ausra
author_sort Drobysh, Maryia
collection PubMed
description In this work, we report an impedimetric system for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein. The sensing platform is based on recombinant Spike protein (SCoV2-rS) immobilized on the phytic acid doped polyaniline films (PANI-PA). The affinity interaction between immobilized SCoV2-rS protein and antibodies in the physiological range of concentrations was registered by electrochemical impedance spectroscopy. Analytical parameters of the sensing platform were tuned by the variation of electropolymerization times during the synthesis of PANI-PA films. The lowest limit of detection and quantification were obtained for electropolymerization time of 20 min and equalled 8.00 ± 0.20 nM and 23.93 ± 0.60 nM with an equilibrium dissociation constant of 3 nM. The presented sensing system is label-free and suitable for the direct detection of antibodies against SARS-CoV-2 in real patient serum samples after coronavirus disease 2019 and/or vaccination.
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spelling pubmed-97262072022-12-07 Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein Drobysh, Maryia Ramanavicius, Arunas Baradoke, Ausra Sci Total Environ Article In this work, we report an impedimetric system for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein. The sensing platform is based on recombinant Spike protein (SCoV2-rS) immobilized on the phytic acid doped polyaniline films (PANI-PA). The affinity interaction between immobilized SCoV2-rS protein and antibodies in the physiological range of concentrations was registered by electrochemical impedance spectroscopy. Analytical parameters of the sensing platform were tuned by the variation of electropolymerization times during the synthesis of PANI-PA films. The lowest limit of detection and quantification were obtained for electropolymerization time of 20 min and equalled 8.00 ± 0.20 nM and 23.93 ± 0.60 nM with an equilibrium dissociation constant of 3 nM. The presented sensing system is label-free and suitable for the direct detection of antibodies against SARS-CoV-2 in real patient serum samples after coronavirus disease 2019 and/or vaccination. Elsevier B.V. 2023-03-01 2022-12-07 /pmc/articles/PMC9726207/ /pubmed/36493838 http://dx.doi.org/10.1016/j.scitotenv.2022.160700 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Drobysh, Maryia
Ramanavicius, Arunas
Baradoke, Ausra
Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein
title Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein
title_full Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein
title_fullStr Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein
title_full_unstemmed Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein
title_short Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein
title_sort polyaniline-based electrochemical immunosensor for the determination of antibodies against sars-cov-2 spike protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726207/
https://www.ncbi.nlm.nih.gov/pubmed/36493838
http://dx.doi.org/10.1016/j.scitotenv.2022.160700
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