Cargando…
Analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: A narrative review
To explore the potential mechanism of triptolide in diabetic nephropathy (DN) treatment using network pharmacology. METHODS: The main targets of triptolide were screened using the TCMSP, DrugBank, and NCBI databases, and gene targets of DN were searched using the DrugBank, DisGeNET, TTD, and OMIM da...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726356/ https://www.ncbi.nlm.nih.gov/pubmed/36482625 http://dx.doi.org/10.1097/MD.0000000000031941 |
_version_ | 1784844761003196416 |
---|---|
author | Gao, Ying Guo, Zhaoan Liu, Yingying |
author_facet | Gao, Ying Guo, Zhaoan Liu, Yingying |
author_sort | Gao, Ying |
collection | PubMed |
description | To explore the potential mechanism of triptolide in diabetic nephropathy (DN) treatment using network pharmacology. METHODS: The main targets of triptolide were screened using the TCMSP, DrugBank, and NCBI databases, and gene targets of DN were searched using the DrugBank, DisGeNET, TTD, and OMIM databases. All of the above targets were normalized using the UniProt database to obtain the co-acting genes. The co-acting genes were uploaded to the STRING platform to build a protein-protein interaction network and screen the core acting targets. Gene ontology and Kyoto encyclopedia of genes and genomes analyses of the core targets were performed using Metascape. Molecular docking validation of triptolide with the co-acting genes was performed using the Swiss Dock platform. RESULTS: We identified 76 potential target points for triptolide, 693 target points for DN-related diseases, and 24 co-acting genes. The main pathways and biological processes involved are lipids and atherosclerosis, IL-18 signaling pathway, TWEAK signaling pathway, response to oxidative stress, hematopoietic function, and negative regulation of cell differentiation. Both triptolide and the active site of the core target genes can form more than 2 hydrogen bonds, and the bond energy is less than -5kJ/mol. Bioinformatics analysis showed that triptolide had a regulatory effect on most of the core target genes that are aberrantly expressed in DKD. CONCLUSION: Triptolide may regulate the body’s response to cytokines, hormones, oxidative stress, and apoptosis signaling pathways in DN treatment by down-regulating Casp3, Casp8, PTEN, GSA3B and up-regulating ESR1, and so forth. |
format | Online Article Text |
id | pubmed-9726356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-97263562022-12-09 Analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: A narrative review Gao, Ying Guo, Zhaoan Liu, Yingying Medicine (Baltimore) 5200 To explore the potential mechanism of triptolide in diabetic nephropathy (DN) treatment using network pharmacology. METHODS: The main targets of triptolide were screened using the TCMSP, DrugBank, and NCBI databases, and gene targets of DN were searched using the DrugBank, DisGeNET, TTD, and OMIM databases. All of the above targets were normalized using the UniProt database to obtain the co-acting genes. The co-acting genes were uploaded to the STRING platform to build a protein-protein interaction network and screen the core acting targets. Gene ontology and Kyoto encyclopedia of genes and genomes analyses of the core targets were performed using Metascape. Molecular docking validation of triptolide with the co-acting genes was performed using the Swiss Dock platform. RESULTS: We identified 76 potential target points for triptolide, 693 target points for DN-related diseases, and 24 co-acting genes. The main pathways and biological processes involved are lipids and atherosclerosis, IL-18 signaling pathway, TWEAK signaling pathway, response to oxidative stress, hematopoietic function, and negative regulation of cell differentiation. Both triptolide and the active site of the core target genes can form more than 2 hydrogen bonds, and the bond energy is less than -5kJ/mol. Bioinformatics analysis showed that triptolide had a regulatory effect on most of the core target genes that are aberrantly expressed in DKD. CONCLUSION: Triptolide may regulate the body’s response to cytokines, hormones, oxidative stress, and apoptosis signaling pathways in DN treatment by down-regulating Casp3, Casp8, PTEN, GSA3B and up-regulating ESR1, and so forth. Lippincott Williams & Wilkins 2022-12-02 /pmc/articles/PMC9726356/ /pubmed/36482625 http://dx.doi.org/10.1097/MD.0000000000031941 Text en Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY) (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | 5200 Gao, Ying Guo, Zhaoan Liu, Yingying Analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: A narrative review |
title | Analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: A narrative review |
title_full | Analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: A narrative review |
title_fullStr | Analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: A narrative review |
title_full_unstemmed | Analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: A narrative review |
title_short | Analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: A narrative review |
title_sort | analysis of the potential molecular biology of triptolide in the treatment of diabetic nephropathy: a narrative review |
topic | 5200 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726356/ https://www.ncbi.nlm.nih.gov/pubmed/36482625 http://dx.doi.org/10.1097/MD.0000000000031941 |
work_keys_str_mv | AT gaoying analysisofthepotentialmolecularbiologyoftriptolideinthetreatmentofdiabeticnephropathyanarrativereview AT guozhaoan analysisofthepotentialmolecularbiologyoftriptolideinthetreatmentofdiabeticnephropathyanarrativereview AT liuyingying analysisofthepotentialmolecularbiologyoftriptolideinthetreatmentofdiabeticnephropathyanarrativereview |