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Next generation genetically encoded fluorescent sensors for serotonin
We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726753/ https://www.ncbi.nlm.nih.gov/pubmed/36473867 http://dx.doi.org/10.1038/s41467-022-35200-w |
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author | Kubitschke, Martin Müller, Monika Wallhorn, Lutz Pulin, Mauro Mittag, Manuel Pollok, Stefan Ziebarth, Tim Bremshey, Svenja Gerdey, Jill Claussen, Kristin Carolin Renken, Kim Groß, Juliana Gneiße, Pascal Meyer, Niklas Wiegert, J. Simon Reiner, Andreas Fuhrmann, Martin Masseck, Olivia Andrea |
author_facet | Kubitschke, Martin Müller, Monika Wallhorn, Lutz Pulin, Mauro Mittag, Manuel Pollok, Stefan Ziebarth, Tim Bremshey, Svenja Gerdey, Jill Claussen, Kristin Carolin Renken, Kim Groß, Juliana Gneiße, Pascal Meyer, Niklas Wiegert, J. Simon Reiner, Andreas Fuhrmann, Martin Masseck, Olivia Andrea |
author_sort | Kubitschke, Martin |
collection | PubMed |
description | We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging. |
format | Online Article Text |
id | pubmed-9726753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-97267532022-12-08 Next generation genetically encoded fluorescent sensors for serotonin Kubitschke, Martin Müller, Monika Wallhorn, Lutz Pulin, Mauro Mittag, Manuel Pollok, Stefan Ziebarth, Tim Bremshey, Svenja Gerdey, Jill Claussen, Kristin Carolin Renken, Kim Groß, Juliana Gneiße, Pascal Meyer, Niklas Wiegert, J. Simon Reiner, Andreas Fuhrmann, Martin Masseck, Olivia Andrea Nat Commun Article We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the versatility in imaging of serotonin dynamics. Experiments in vitro and in vivo showed the feasibility of imaging serotonin dynamics with high temporal and spatial resolution. As demonstrated here, the designed sensors show excellent membrane expression, have high specificity and a superior signal-to-noise ratio, detect the endogenous release of serotonin and are suitable for two-photon in vivo imaging. Nature Publishing Group UK 2022-12-06 /pmc/articles/PMC9726753/ /pubmed/36473867 http://dx.doi.org/10.1038/s41467-022-35200-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kubitschke, Martin Müller, Monika Wallhorn, Lutz Pulin, Mauro Mittag, Manuel Pollok, Stefan Ziebarth, Tim Bremshey, Svenja Gerdey, Jill Claussen, Kristin Carolin Renken, Kim Groß, Juliana Gneiße, Pascal Meyer, Niklas Wiegert, J. Simon Reiner, Andreas Fuhrmann, Martin Masseck, Olivia Andrea Next generation genetically encoded fluorescent sensors for serotonin |
title | Next generation genetically encoded fluorescent sensors for serotonin |
title_full | Next generation genetically encoded fluorescent sensors for serotonin |
title_fullStr | Next generation genetically encoded fluorescent sensors for serotonin |
title_full_unstemmed | Next generation genetically encoded fluorescent sensors for serotonin |
title_short | Next generation genetically encoded fluorescent sensors for serotonin |
title_sort | next generation genetically encoded fluorescent sensors for serotonin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726753/ https://www.ncbi.nlm.nih.gov/pubmed/36473867 http://dx.doi.org/10.1038/s41467-022-35200-w |
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