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Next generation genetically encoded fluorescent sensors for serotonin

We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities...

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Detalles Bibliográficos
Autores principales:	Kubitschke, Martin, Müller, Monika, Wallhorn, Lutz, Pulin, Mauro, Mittag, Manuel, Pollok, Stefan, Ziebarth, Tim, Bremshey, Svenja, Gerdey, Jill, Claussen, Kristin Carolin, Renken, Kim, Groß, Juliana, Gneiße, Pascal, Meyer, Niklas, Wiegert, J. Simon, Reiner, Andreas, Fuhrmann, Martin, Masseck, Olivia Andrea
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9726753/ https://www.ncbi.nlm.nih.gov/pubmed/36473867 http://dx.doi.org/10.1038/s41467-022-35200-w

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