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Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study

Coccidiosis, an acute epidemic intestinal disease of poultry, is caused by the parasitic protozoan genus Eimeria, with Eimeria tenella being the most pathogenic spp. Novel approaches are required to address the limitations of current treatments for this disease. We investigated the effects of eight...

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Autores principales: Han, Mingzheng, Hu, Wenfeng, Chen, Tong, Guo, Hanxing, Zhu, Jianfeng, Chen, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727100/
https://www.ncbi.nlm.nih.gov/pubmed/36504841
http://dx.doi.org/10.3389/fvets.2022.1066543
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author Han, Mingzheng
Hu, Wenfeng
Chen, Tong
Guo, Hanxing
Zhu, Jianfeng
Chen, Feng
author_facet Han, Mingzheng
Hu, Wenfeng
Chen, Tong
Guo, Hanxing
Zhu, Jianfeng
Chen, Feng
author_sort Han, Mingzheng
collection PubMed
description Coccidiosis, an acute epidemic intestinal disease of poultry, is caused by the parasitic protozoan genus Eimeria, with Eimeria tenella being the most pathogenic spp. Novel approaches are required to address the limitations of current treatments for this disease. We investigated the effects of eight plant extracts and essential oils and their mixture on Eimeria tenella as potential treatments for coccidial infection. The anticoccidial effects of non-toxic concentrations of Punica granatum L. (0.005 mg/mL), Plantago asiatica L. (0.780 mg/mL), Bidens pilosa L. (0.390 mg/mL), Acalypha australis L. (0.390 mg/mL), Pteris multifida Poir (0.050 mg/mL), and Portulaca oleracea L. sp. Pl. (0.050 mg/mL) extracts; Artemisia argyi Levl. et Vant. (0.010 μL/mL) and Camellia sinensis (L.) O. Ktze (0.050 μL/mL) essential oils; and their mixture (0.500 mL/mL) on Eimeria tenella were determined using cell viability assays, flow cytometry, and in vivo studies. The eight plant extracts and essential oils and their mixture inhibited Eimeria tenella sporozoites from invading chicken embryo fibroblast cells in vitro. The extract and essential oil mixture improved the feed conversion ratio and body weight gain, reduced fecal oocyst excretion, substantially reduced the mortality of Eimeria tenella-infected chickens, and reduced Eimeria tenella-induced cecal damage in vivo. The results suggest that the extract and essential oil mixtures inhibit Eimeria tenella invasion both in vitro and in vivo, demonstrating their potential as anticoccidial agents.
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spelling pubmed-97271002022-12-08 Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study Han, Mingzheng Hu, Wenfeng Chen, Tong Guo, Hanxing Zhu, Jianfeng Chen, Feng Front Vet Sci Veterinary Science Coccidiosis, an acute epidemic intestinal disease of poultry, is caused by the parasitic protozoan genus Eimeria, with Eimeria tenella being the most pathogenic spp. Novel approaches are required to address the limitations of current treatments for this disease. We investigated the effects of eight plant extracts and essential oils and their mixture on Eimeria tenella as potential treatments for coccidial infection. The anticoccidial effects of non-toxic concentrations of Punica granatum L. (0.005 mg/mL), Plantago asiatica L. (0.780 mg/mL), Bidens pilosa L. (0.390 mg/mL), Acalypha australis L. (0.390 mg/mL), Pteris multifida Poir (0.050 mg/mL), and Portulaca oleracea L. sp. Pl. (0.050 mg/mL) extracts; Artemisia argyi Levl. et Vant. (0.010 μL/mL) and Camellia sinensis (L.) O. Ktze (0.050 μL/mL) essential oils; and their mixture (0.500 mL/mL) on Eimeria tenella were determined using cell viability assays, flow cytometry, and in vivo studies. The eight plant extracts and essential oils and their mixture inhibited Eimeria tenella sporozoites from invading chicken embryo fibroblast cells in vitro. The extract and essential oil mixture improved the feed conversion ratio and body weight gain, reduced fecal oocyst excretion, substantially reduced the mortality of Eimeria tenella-infected chickens, and reduced Eimeria tenella-induced cecal damage in vivo. The results suggest that the extract and essential oil mixtures inhibit Eimeria tenella invasion both in vitro and in vivo, demonstrating their potential as anticoccidial agents. Frontiers Media S.A. 2022-11-23 /pmc/articles/PMC9727100/ /pubmed/36504841 http://dx.doi.org/10.3389/fvets.2022.1066543 Text en Copyright © 2022 Han, Hu, Chen, Guo, Zhu and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Han, Mingzheng
Hu, Wenfeng
Chen, Tong
Guo, Hanxing
Zhu, Jianfeng
Chen, Feng
Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study
title Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study
title_full Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study
title_fullStr Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study
title_full_unstemmed Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study
title_short Anticoccidial activity of natural plants extracts mixture against Eimeria tenella: An in vitro and in vivo study
title_sort anticoccidial activity of natural plants extracts mixture against eimeria tenella: an in vitro and in vivo study
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727100/
https://www.ncbi.nlm.nih.gov/pubmed/36504841
http://dx.doi.org/10.3389/fvets.2022.1066543
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