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Scale-up of biomass production by Methanococcus maripaludis

The development of a sustainable energy economy is one of the great challenges in the current times of climate crisis and growing energy demands. Industrial production of the fifth-generation biofuel methane by microorganisms has the potential to become a crucial biotechnological milestone of the po...

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Autores principales: Palabikyan, Hayk, Ruddyard, Aquilla, Pomper, Lara, Novak, David, Reischl, Barbara, Rittmann, Simon K.-M. R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727139/
https://www.ncbi.nlm.nih.gov/pubmed/36504798
http://dx.doi.org/10.3389/fmicb.2022.1031131
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author Palabikyan, Hayk
Ruddyard, Aquilla
Pomper, Lara
Novak, David
Reischl, Barbara
Rittmann, Simon K.-M. R.
author_facet Palabikyan, Hayk
Ruddyard, Aquilla
Pomper, Lara
Novak, David
Reischl, Barbara
Rittmann, Simon K.-M. R.
author_sort Palabikyan, Hayk
collection PubMed
description The development of a sustainable energy economy is one of the great challenges in the current times of climate crisis and growing energy demands. Industrial production of the fifth-generation biofuel methane by microorganisms has the potential to become a crucial biotechnological milestone of the post fossil fuel era. Therefore, reproducible cultivation and scale-up of methanogenic archaea (methanogens) is essential for enabling biomass generation for fundamental studies and for defining peak performance conditions for bioprocess development. This study provides a comprehensive revision of established and optimization of novel methods for the cultivation of the model organism Methanococcus maripaludis S0001. In closed batch mode, 0.05 L serum bottles cultures were gradually replaced by 0.4 L Schott bottle cultures for regular biomass generation, and the time for reaching peak optical density (OD(578)) values was reduced in half. In 1.5 L reactor cultures, various agitation, harvesting and transfer methods were compared resulting in a specific growth rate of 0.16 h(−1) and the highest recorded OD(578) of 3.4. Finally, a 300-fold scale-up from serum bottles was achieved by growing M. maripaludis for the first time in a 22 L stainless steel bioreactor with 15 L working volume. Altogether, the experimental approaches described in this study contribute to establishing methanogens as essential organisms in large-scale biotechnology applications, a crucial stage of an urgently needed industrial evolution toward sustainable biosynthesis of energy and high value products.
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spelling pubmed-97271392022-12-08 Scale-up of biomass production by Methanococcus maripaludis Palabikyan, Hayk Ruddyard, Aquilla Pomper, Lara Novak, David Reischl, Barbara Rittmann, Simon K.-M. R. Front Microbiol Microbiology The development of a sustainable energy economy is one of the great challenges in the current times of climate crisis and growing energy demands. Industrial production of the fifth-generation biofuel methane by microorganisms has the potential to become a crucial biotechnological milestone of the post fossil fuel era. Therefore, reproducible cultivation and scale-up of methanogenic archaea (methanogens) is essential for enabling biomass generation for fundamental studies and for defining peak performance conditions for bioprocess development. This study provides a comprehensive revision of established and optimization of novel methods for the cultivation of the model organism Methanococcus maripaludis S0001. In closed batch mode, 0.05 L serum bottles cultures were gradually replaced by 0.4 L Schott bottle cultures for regular biomass generation, and the time for reaching peak optical density (OD(578)) values was reduced in half. In 1.5 L reactor cultures, various agitation, harvesting and transfer methods were compared resulting in a specific growth rate of 0.16 h(−1) and the highest recorded OD(578) of 3.4. Finally, a 300-fold scale-up from serum bottles was achieved by growing M. maripaludis for the first time in a 22 L stainless steel bioreactor with 15 L working volume. Altogether, the experimental approaches described in this study contribute to establishing methanogens as essential organisms in large-scale biotechnology applications, a crucial stage of an urgently needed industrial evolution toward sustainable biosynthesis of energy and high value products. Frontiers Media S.A. 2022-11-23 /pmc/articles/PMC9727139/ /pubmed/36504798 http://dx.doi.org/10.3389/fmicb.2022.1031131 Text en Copyright © 2022 Palabikyan, Ruddyard, Pomper, Novak, Reischl and Rittmann. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Palabikyan, Hayk
Ruddyard, Aquilla
Pomper, Lara
Novak, David
Reischl, Barbara
Rittmann, Simon K.-M. R.
Scale-up of biomass production by Methanococcus maripaludis
title Scale-up of biomass production by Methanococcus maripaludis
title_full Scale-up of biomass production by Methanococcus maripaludis
title_fullStr Scale-up of biomass production by Methanococcus maripaludis
title_full_unstemmed Scale-up of biomass production by Methanococcus maripaludis
title_short Scale-up of biomass production by Methanococcus maripaludis
title_sort scale-up of biomass production by methanococcus maripaludis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727139/
https://www.ncbi.nlm.nih.gov/pubmed/36504798
http://dx.doi.org/10.3389/fmicb.2022.1031131
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