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ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2

Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown to involve in the splicing of different mitocho...

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Autores principales: Fan, Kaijian, Fu, Qinghui, Wei, Qianhan, Jia, Sinian, Zhao, Anqi, Wang, Tengteng, Cao, Jie, Liu, Yan, Ren, Zhenjing, Liu, Yunjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727264/
https://www.ncbi.nlm.nih.gov/pubmed/36507372
http://dx.doi.org/10.3389/fpls.2022.1033869
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author Fan, Kaijian
Fu, Qinghui
Wei, Qianhan
Jia, Sinian
Zhao, Anqi
Wang, Tengteng
Cao, Jie
Liu, Yan
Ren, Zhenjing
Liu, Yunjun
author_facet Fan, Kaijian
Fu, Qinghui
Wei, Qianhan
Jia, Sinian
Zhao, Anqi
Wang, Tengteng
Cao, Jie
Liu, Yan
Ren, Zhenjing
Liu, Yunjun
author_sort Fan, Kaijian
collection PubMed
description Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown to involve in the splicing of different mitochondrial group II introns; however, the specific biological functions of maturases in maize are largely uncharacterized. In this study, we identified a maize ZmnMAT1 gene, which encodes a mitochondrion-localized type I maturase with an RT domain at N-terminus and an X domain at C-terminus. Loss-of-function mutation in ZmnMAT1 significantly reduced the splicing efficiencies of Nad1 intron 1 and Nad4 intron 2, and showed arrested embryogenesis and endosperm development, which may be related to impaired mitochondrial ultrastructure and function due to the destruction of the assembly and activity of complex I. Direct physical interaction was undetectable between ZmnMAT1 and the proteins associated with the splicing of Nad1 intron 1 and/or Nad4 intron 2 by yeast two-hybrid assays, suggesting the complexity of group II intron splicing in plants.
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spelling pubmed-97272642022-12-08 ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2 Fan, Kaijian Fu, Qinghui Wei, Qianhan Jia, Sinian Zhao, Anqi Wang, Tengteng Cao, Jie Liu, Yan Ren, Zhenjing Liu, Yunjun Front Plant Sci Plant Science Maturases can specifically bind to intron-containing pre-RNAs, folding them into catalytic structures that facilitate intron splicing in vivo. Plants possess four nuclear-encoded maturase-related factors (nMAT1-nMAT4) and some maturases have been shown to involve in the splicing of different mitochondrial group II introns; however, the specific biological functions of maturases in maize are largely uncharacterized. In this study, we identified a maize ZmnMAT1 gene, which encodes a mitochondrion-localized type I maturase with an RT domain at N-terminus and an X domain at C-terminus. Loss-of-function mutation in ZmnMAT1 significantly reduced the splicing efficiencies of Nad1 intron 1 and Nad4 intron 2, and showed arrested embryogenesis and endosperm development, which may be related to impaired mitochondrial ultrastructure and function due to the destruction of the assembly and activity of complex I. Direct physical interaction was undetectable between ZmnMAT1 and the proteins associated with the splicing of Nad1 intron 1 and/or Nad4 intron 2 by yeast two-hybrid assays, suggesting the complexity of group II intron splicing in plants. Frontiers Media S.A. 2022-11-23 /pmc/articles/PMC9727264/ /pubmed/36507372 http://dx.doi.org/10.3389/fpls.2022.1033869 Text en Copyright © 2022 Fan, Fu, Wei, Jia, Zhao, Wang, Cao, Liu, Ren and Liu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Fan, Kaijian
Fu, Qinghui
Wei, Qianhan
Jia, Sinian
Zhao, Anqi
Wang, Tengteng
Cao, Jie
Liu, Yan
Ren, Zhenjing
Liu, Yunjun
ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2
title ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2
title_full ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2
title_fullStr ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2
title_full_unstemmed ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2
title_short ZmnMAT1, a nuclear-encoded type I maturase, is required for the splicing of mitochondrial Nad1 intron 1 and Nad4 intron 2
title_sort zmnmat1, a nuclear-encoded type i maturase, is required for the splicing of mitochondrial nad1 intron 1 and nad4 intron 2
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727264/
https://www.ncbi.nlm.nih.gov/pubmed/36507372
http://dx.doi.org/10.3389/fpls.2022.1033869
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