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Genome variability of domestic tomato varieties: data from AFLP analysis

Tomato Solanum lycopersicum L. is one of the main vegetable crops, accessions and cultivars of which are characterized by a low level of genomic polymorphism. Introgressive tomato breeding uses related wild Solanum species to improve cultivars for stress tolerance and fruit quality traits. The aim o...

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Autores principales: Kulakova, A.V., Dyachenko, E.A., Shchennikova, A.V., Pyshnaya, O.N., Dzhos, E.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727544/
https://www.ncbi.nlm.nih.gov/pubmed/36532629
http://dx.doi.org/10.18699/VJGB-22-80
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author Kulakova, A.V.
Dyachenko, E.A.
Shchennikova, A.V.
Pyshnaya, O.N.
Dzhos, E.A.
author_facet Kulakova, A.V.
Dyachenko, E.A.
Shchennikova, A.V.
Pyshnaya, O.N.
Dzhos, E.A.
author_sort Kulakova, A.V.
collection PubMed
description Tomato Solanum lycopersicum L. is one of the main vegetable crops, accessions and cultivars of which are characterized by a low level of genomic polymorphism. Introgressive tomato breeding uses related wild Solanum species to improve cultivars for stress tolerance and fruit quality traits. The aim of this work was to evaluate the genome variability of 59 cultivars and perspective breeding lines of S. lycopersicum and 11 wild tomato species using the AFLP method. According to the AFLP analysis, four combinations of primers E32/M59, E32/M57, E38/M57, and E41/M59, which had the highest PIC (polymorphism information content) values, were selected. In the process of genotyping a collection of 59 cultivars/lines of S. lycopersicum and 11 wild tomato accessions, the selected primers revealed 391 fragments ranging in size from 80 to 450 bp, of which 114 fragments turned out to be polymorphic and 25 were unique. Analysis of the amplif ication spectra placed wild tomato accessions into separate clades. Sister clades included cultivars of FSCV breeding resistant to drought and/or cold and, in part, to late blight, Alternaria, Septoria, tobacco mosaic virus and blossom end rot, as well as tomato accessions not characterized according to these traits, which suggests that they have resistance to stress factors. In accessions of distant clades, there was clustering on the basis of resistance to Verticillium, cladosporiosis, Fusarium, tobacco mosaic virus, gray rot, and blossom end rot. The combination of ac cessions according to their origin from the originating organization was shown. The primer combinations E32/M59, E32/M57, E38/M57 and E41/M59 were shown to be perspective for genotyping tomato cultivars to select donors of resistance to various stress factors. The clade-specif ic fragments identif ied in this work can become the basis for the development of AFLP markers for traits of resistance to stress factors.
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spelling pubmed-97275442022-12-16 Genome variability of domestic tomato varieties: data from AFLP analysis Kulakova, A.V. Dyachenko, E.A. Shchennikova, A.V. Pyshnaya, O.N. Dzhos, E.A. Vavilovskii Zhurnal Genet Selektsii Original Article Tomato Solanum lycopersicum L. is one of the main vegetable crops, accessions and cultivars of which are characterized by a low level of genomic polymorphism. Introgressive tomato breeding uses related wild Solanum species to improve cultivars for stress tolerance and fruit quality traits. The aim of this work was to evaluate the genome variability of 59 cultivars and perspective breeding lines of S. lycopersicum and 11 wild tomato species using the AFLP method. According to the AFLP analysis, four combinations of primers E32/M59, E32/M57, E38/M57, and E41/M59, which had the highest PIC (polymorphism information content) values, were selected. In the process of genotyping a collection of 59 cultivars/lines of S. lycopersicum and 11 wild tomato accessions, the selected primers revealed 391 fragments ranging in size from 80 to 450 bp, of which 114 fragments turned out to be polymorphic and 25 were unique. Analysis of the amplif ication spectra placed wild tomato accessions into separate clades. Sister clades included cultivars of FSCV breeding resistant to drought and/or cold and, in part, to late blight, Alternaria, Septoria, tobacco mosaic virus and blossom end rot, as well as tomato accessions not characterized according to these traits, which suggests that they have resistance to stress factors. In accessions of distant clades, there was clustering on the basis of resistance to Verticillium, cladosporiosis, Fusarium, tobacco mosaic virus, gray rot, and blossom end rot. The combination of ac cessions according to their origin from the originating organization was shown. The primer combinations E32/M59, E32/M57, E38/M57 and E41/M59 were shown to be perspective for genotyping tomato cultivars to select donors of resistance to various stress factors. The clade-specif ic fragments identif ied in this work can become the basis for the development of AFLP markers for traits of resistance to stress factors. The Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2022-11 /pmc/articles/PMC9727544/ /pubmed/36532629 http://dx.doi.org/10.18699/VJGB-22-80 Text en Copyright © AUTHORS https://creativecommons.org/licenses/by/2.5/This work is licensed under a Creative Commons Attribution 4.0 License
spellingShingle Original Article
Kulakova, A.V.
Dyachenko, E.A.
Shchennikova, A.V.
Pyshnaya, O.N.
Dzhos, E.A.
Genome variability of domestic tomato varieties: data from AFLP analysis
title Genome variability of domestic tomato varieties: data from AFLP analysis
title_full Genome variability of domestic tomato varieties: data from AFLP analysis
title_fullStr Genome variability of domestic tomato varieties: data from AFLP analysis
title_full_unstemmed Genome variability of domestic tomato varieties: data from AFLP analysis
title_short Genome variability of domestic tomato varieties: data from AFLP analysis
title_sort genome variability of domestic tomato varieties: data from aflp analysis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727544/
https://www.ncbi.nlm.nih.gov/pubmed/36532629
http://dx.doi.org/10.18699/VJGB-22-80
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