Cargando…
KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC
Kinesin-driven organelle transport is crucial for neuron development and maintenance, yet the mechanisms by which kinesins specifically bind their organelle cargoes remain undefined. In contrast to other transport kinesins, the neuronal function and specific organelle adaptors of heterodimeric Kines...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727798/ https://www.ncbi.nlm.nih.gov/pubmed/36200888 http://dx.doi.org/10.1091/mbc.E22-08-0336 |
_version_ | 1784845103078047744 |
---|---|
author | Garbouchian, Alex Montgomery, Andrew C. Gilbert, Susan P. Bentley, Marvin |
author_facet | Garbouchian, Alex Montgomery, Andrew C. Gilbert, Susan P. Bentley, Marvin |
author_sort | Garbouchian, Alex |
collection | PubMed |
description | Kinesin-driven organelle transport is crucial for neuron development and maintenance, yet the mechanisms by which kinesins specifically bind their organelle cargoes remain undefined. In contrast to other transport kinesins, the neuronal function and specific organelle adaptors of heterodimeric Kinesin-2 family members KIF3AB and KIF3AC remain unknown. We developed a novel microscopy-based assay to define protein–protein interactions in intact neurons. The experiments found that both KIF3AB and KIF3AC bind kinesin-associated protein (KAP). These interactions are mediated by the distal C-terminal tail regions and not the coiled-coil domain. We used live-cell imaging in cultured hippocampal neurons to define the localization and trafficking parameters of KIF3AB and KIF3AC organelle populations. We discovered that KIF3AB/KAP and KIF3AC/KAP bind the same organelle populations and defined their transport parameters in axons and dendrites. The results also show that ∼12% of KIF3 organelles contain the RNA-binding protein adenomatous polyposis coli. These data point toward a model in which KIF3AB and KIF3AC use KAP as their neuronal organelle adaptor and that these kinesins mediate transport of a range of organelles. |
format | Online Article Text |
id | pubmed-9727798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97277982023-02-02 KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC Garbouchian, Alex Montgomery, Andrew C. Gilbert, Susan P. Bentley, Marvin Mol Biol Cell Articles Kinesin-driven organelle transport is crucial for neuron development and maintenance, yet the mechanisms by which kinesins specifically bind their organelle cargoes remain undefined. In contrast to other transport kinesins, the neuronal function and specific organelle adaptors of heterodimeric Kinesin-2 family members KIF3AB and KIF3AC remain unknown. We developed a novel microscopy-based assay to define protein–protein interactions in intact neurons. The experiments found that both KIF3AB and KIF3AC bind kinesin-associated protein (KAP). These interactions are mediated by the distal C-terminal tail regions and not the coiled-coil domain. We used live-cell imaging in cultured hippocampal neurons to define the localization and trafficking parameters of KIF3AB and KIF3AC organelle populations. We discovered that KIF3AB/KAP and KIF3AC/KAP bind the same organelle populations and defined their transport parameters in axons and dendrites. The results also show that ∼12% of KIF3 organelles contain the RNA-binding protein adenomatous polyposis coli. These data point toward a model in which KIF3AB and KIF3AC use KAP as their neuronal organelle adaptor and that these kinesins mediate transport of a range of organelles. The American Society for Cell Biology 2022-11-18 /pmc/articles/PMC9727798/ /pubmed/36200888 http://dx.doi.org/10.1091/mbc.E22-08-0336 Text en © 2022 Garbouchian et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License. |
spellingShingle | Articles Garbouchian, Alex Montgomery, Andrew C. Gilbert, Susan P. Bentley, Marvin KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC |
title | KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC |
title_full | KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC |
title_fullStr | KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC |
title_full_unstemmed | KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC |
title_short | KAP is the neuronal organelle adaptor for Kinesin-2 KIF3AB and KIF3AC |
title_sort | kap is the neuronal organelle adaptor for kinesin-2 kif3ab and kif3ac |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9727798/ https://www.ncbi.nlm.nih.gov/pubmed/36200888 http://dx.doi.org/10.1091/mbc.E22-08-0336 |
work_keys_str_mv | AT garbouchianalex kapistheneuronalorganelleadaptorforkinesin2kif3abandkif3ac AT montgomeryandrewc kapistheneuronalorganelleadaptorforkinesin2kif3abandkif3ac AT gilbertsusanp kapistheneuronalorganelleadaptorforkinesin2kif3abandkif3ac AT bentleymarvin kapistheneuronalorganelleadaptorforkinesin2kif3abandkif3ac |