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Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract

Panax ginseng has a wide range of activities including a neuroprotective effect, skin protective effects, enhanced DNA repairing, anti-diabetic activity, and protective effects against vascular inflammation. In the present study, we sought to discover the inhibitory effects of a mixture of natural p...

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Autores principales: Li, Liang, Park, Young-Ran, Shrestha, Saroj Kumar, Cho, Hyoung-Kwon, Soh, Yunjo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Microbiology and Biotechnology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728228/
https://www.ncbi.nlm.nih.gov/pubmed/32807758
http://dx.doi.org/10.4014/jmb.2004.04016
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author Li, Liang
Park, Young-Ran
Shrestha, Saroj Kumar
Cho, Hyoung-Kwon
Soh, Yunjo
author_facet Li, Liang
Park, Young-Ran
Shrestha, Saroj Kumar
Cho, Hyoung-Kwon
Soh, Yunjo
author_sort Li, Liang
collection PubMed
description Panax ginseng has a wide range of activities including a neuroprotective effect, skin protective effects, enhanced DNA repairing, anti-diabetic activity, and protective effects against vascular inflammation. In the present study, we sought to discover the inhibitory effects of a mixture of natural products containing Panax ginseng, Ziziphus jujube, Rubi fructus, Artemisiae asiaticae and Scutellaria baicalensis (PZRAS) on osteoclastogenesis and bone remodeling, as neither the effects of a mixture containing Panax ginseng extract, nor its molecular mechanism on bone inflammation, have been clarified yet. PZRAS upregulated the levels of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GSH-R) and glutathione peroxidase (GSH-Px) and reduced malondialdehyde (MDA) in LPS-treated RAW264.7 cells. Moreover, treatment with PZRAS decreased the production of IL-1β and TNF-α. PZRAS also inhibited osteoclast differentiation through inhibiting osteoclastspecific genes like MMP-2, 9, cathepsin K, and TRAP in RANKL-treated RAW264.7 cells. Additionally, PZRAS has inhibitory functions on the RANKL-stimulated activation of ERK and JNK, which lead to a decrease in the expression of NFATc1 and c-Fos. In an in vivo study, bone resorption induced by LPS was recovered by treatment with PZRAS in bone volume per tissue volume (BV/TV) compared to control. Furthermore, the ratio of eroded bone surface of femurs was significantly increased in LPStreated mice compared to vehicle group, but this ratio was significantly reversed in PZRAS-treated mice. These results suggest that PZRAS could prevent or treat disorders with abnormal bone loss.
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spelling pubmed-97282282022-12-13 Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract Li, Liang Park, Young-Ran Shrestha, Saroj Kumar Cho, Hyoung-Kwon Soh, Yunjo J Microbiol Biotechnol Research article Panax ginseng has a wide range of activities including a neuroprotective effect, skin protective effects, enhanced DNA repairing, anti-diabetic activity, and protective effects against vascular inflammation. In the present study, we sought to discover the inhibitory effects of a mixture of natural products containing Panax ginseng, Ziziphus jujube, Rubi fructus, Artemisiae asiaticae and Scutellaria baicalensis (PZRAS) on osteoclastogenesis and bone remodeling, as neither the effects of a mixture containing Panax ginseng extract, nor its molecular mechanism on bone inflammation, have been clarified yet. PZRAS upregulated the levels of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GSH-R) and glutathione peroxidase (GSH-Px) and reduced malondialdehyde (MDA) in LPS-treated RAW264.7 cells. Moreover, treatment with PZRAS decreased the production of IL-1β and TNF-α. PZRAS also inhibited osteoclast differentiation through inhibiting osteoclastspecific genes like MMP-2, 9, cathepsin K, and TRAP in RANKL-treated RAW264.7 cells. Additionally, PZRAS has inhibitory functions on the RANKL-stimulated activation of ERK and JNK, which lead to a decrease in the expression of NFATc1 and c-Fos. In an in vivo study, bone resorption induced by LPS was recovered by treatment with PZRAS in bone volume per tissue volume (BV/TV) compared to control. Furthermore, the ratio of eroded bone surface of femurs was significantly increased in LPStreated mice compared to vehicle group, but this ratio was significantly reversed in PZRAS-treated mice. These results suggest that PZRAS could prevent or treat disorders with abnormal bone loss. Korean Society for Microbiology and Biotechnology 2020-10-28 2020-08-15 /pmc/articles/PMC9728228/ /pubmed/32807758 http://dx.doi.org/10.4014/jmb.2004.04016 Text en Copyright©2020 by The Korean Society for Microbiology and Biotechnology https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Li, Liang
Park, Young-Ran
Shrestha, Saroj Kumar
Cho, Hyoung-Kwon
Soh, Yunjo
Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract
title Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract
title_full Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract
title_fullStr Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract
title_full_unstemmed Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract
title_short Suppression of Inflammation, Osteoclastogenesis and Bone Loss by PZRAS Extract
title_sort suppression of inflammation, osteoclastogenesis and bone loss by pzras extract
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728228/
https://www.ncbi.nlm.nih.gov/pubmed/32807758
http://dx.doi.org/10.4014/jmb.2004.04016
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