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Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice

In this study, we investigated the effects of linoleic acid (LA) treatment on Brucella abortus infection in professional phagocyte RAW264.7 cells, particularly during the pathogen’s invasion and intracellular growth in these cells, as well as in murine model BALB/c mice focusing on bacterial splenic...

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Autores principales: Reyes, Alisha Wehdnesday Bernardo, Vu, Son Hai, Huy, Tran Xuan Ngoc, Min, Wongi, Lee, Hu Jang, Chang, Hong Hee, Lee, John Hwa, Kim, Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Microbiology and Biotechnology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728246/
https://www.ncbi.nlm.nih.gov/pubmed/32482929
http://dx.doi.org/10.4014/jmb.1911.11037
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author Reyes, Alisha Wehdnesday Bernardo
Vu, Son Hai
Huy, Tran Xuan Ngoc
Min, Wongi
Lee, Hu Jang
Chang, Hong Hee
Lee, John Hwa
Kim, Suk
author_facet Reyes, Alisha Wehdnesday Bernardo
Vu, Son Hai
Huy, Tran Xuan Ngoc
Min, Wongi
Lee, Hu Jang
Chang, Hong Hee
Lee, John Hwa
Kim, Suk
author_sort Reyes, Alisha Wehdnesday Bernardo
collection PubMed
description In this study, we investigated the effects of linoleic acid (LA) treatment on Brucella abortus infection in professional phagocyte RAW264.7 cells, particularly during the pathogen’s invasion and intracellular growth in these cells, as well as in murine model BALB/c mice focusing on bacterial splenic proliferation and immunoregulatory activities. LA inhibited the growth of Brucella in a doseand time-dependent manner. The ability of the pathogen to enter the phagocytes was inhibited as was its survival within these cells. This was accompanied by increased nitrite accumulation in these cells at 24 h post-infection. The concentration of LA used in the present study did not affect the total body weight or liver function of the mice. During Brucella infection, the total splenic weight of these animals was not changed; rather, resistance to bacterial proliferation was enhanced in the spleen. Furthermore, mice treated with LA displayed elevated levels of IL-12 and IFN-γ but reduced levels of IL-10 during infection. The findings in this study showed the regulatory role of LA against B. abortus infection suggesting its potential use in designing intervention strategy for brucellosis.
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spelling pubmed-97282462022-12-13 Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice Reyes, Alisha Wehdnesday Bernardo Vu, Son Hai Huy, Tran Xuan Ngoc Min, Wongi Lee, Hu Jang Chang, Hong Hee Lee, John Hwa Kim, Suk J Microbiol Biotechnol Research article In this study, we investigated the effects of linoleic acid (LA) treatment on Brucella abortus infection in professional phagocyte RAW264.7 cells, particularly during the pathogen’s invasion and intracellular growth in these cells, as well as in murine model BALB/c mice focusing on bacterial splenic proliferation and immunoregulatory activities. LA inhibited the growth of Brucella in a doseand time-dependent manner. The ability of the pathogen to enter the phagocytes was inhibited as was its survival within these cells. This was accompanied by increased nitrite accumulation in these cells at 24 h post-infection. The concentration of LA used in the present study did not affect the total body weight or liver function of the mice. During Brucella infection, the total splenic weight of these animals was not changed; rather, resistance to bacterial proliferation was enhanced in the spleen. Furthermore, mice treated with LA displayed elevated levels of IL-12 and IFN-γ but reduced levels of IL-10 during infection. The findings in this study showed the regulatory role of LA against B. abortus infection suggesting its potential use in designing intervention strategy for brucellosis. Korean Society for Microbiology and Biotechnology 2020-05-28 2020-02-05 /pmc/articles/PMC9728246/ /pubmed/32482929 http://dx.doi.org/10.4014/jmb.1911.11037 Text en Copyright©2020 by The Korean Society for Microbiology and Biotechnology https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Reyes, Alisha Wehdnesday Bernardo
Vu, Son Hai
Huy, Tran Xuan Ngoc
Min, Wongi
Lee, Hu Jang
Chang, Hong Hee
Lee, John Hwa
Kim, Suk
Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice
title Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice
title_full Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice
title_fullStr Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice
title_full_unstemmed Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice
title_short Modulatory Effect of Linoleic Acid During Brucella abortus 544 Infection in Murine Macrophage RAW264.7 Cells and Murine Model BALB/c Mice
title_sort modulatory effect of linoleic acid during brucella abortus 544 infection in murine macrophage raw264.7 cells and murine model balb/c mice
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728246/
https://www.ncbi.nlm.nih.gov/pubmed/32482929
http://dx.doi.org/10.4014/jmb.1911.11037
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