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Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake

Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern A...

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Autores principales: Lee, Nan-Yeong, Choi, Doo-Ho, Kim, Mi-Gyeong, Jeong, Min-Ji, Kwon, Hae-Jun, Kim, Dong-Hyun, Kim, Young-Guk, Luccio, Eric di, Arioka, Manabu, Yoon, Hyeok-Jun, Kim, Jong-Guk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Microbiology and Biotechnology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728333/
https://www.ncbi.nlm.nih.gov/pubmed/32120462
http://dx.doi.org/10.4014/jmb.2001.01049
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author Lee, Nan-Yeong
Choi, Doo-Ho
Kim, Mi-Gyeong
Jeong, Min-Ji
Kwon, Hae-Jun
Kim, Dong-Hyun
Kim, Young-Guk
Luccio, Eric di
Arioka, Manabu
Yoon, Hyeok-Jun
Kim, Jong-Guk
author_facet Lee, Nan-Yeong
Choi, Doo-Ho
Kim, Mi-Gyeong
Jeong, Min-Ji
Kwon, Hae-Jun
Kim, Dong-Hyun
Kim, Young-Guk
Luccio, Eric di
Arioka, Manabu
Yoon, Hyeok-Jun
Kim, Jong-Guk
author_sort Lee, Nan-Yeong
collection PubMed
description Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward.
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spelling pubmed-97283332022-12-13 Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake Lee, Nan-Yeong Choi, Doo-Ho Kim, Mi-Gyeong Jeong, Min-Ji Kwon, Hae-Jun Kim, Dong-Hyun Kim, Young-Guk Luccio, Eric di Arioka, Manabu Yoon, Hyeok-Jun Kim, Jong-Guk J Microbiol Biotechnol Research article Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward. Korean Society for Microbiology and Biotechnology 2020-02-28 2020-02-17 /pmc/articles/PMC9728333/ /pubmed/32120462 http://dx.doi.org/10.4014/jmb.2001.01049 Text en Copyright©2020 by The Korean Society for Microbiology and Biotechnology https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Lee, Nan-Yeong
Choi, Doo-Ho
Kim, Mi-Gyeong
Jeong, Min-Ji
Kwon, Hae-Jun
Kim, Dong-Hyun
Kim, Young-Guk
Luccio, Eric di
Arioka, Manabu
Yoon, Hyeok-Jun
Kim, Jong-Guk
Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
title Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
title_full Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
title_fullStr Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
title_full_unstemmed Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
title_short Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
title_sort biosynthesis of (r)-(-)-1-octen-3-ol in recombinant saccharomyces cerevisiae with lipoxygenase-1 and hydroperoxide lyase genes from tricholoma matsutake
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728333/
https://www.ncbi.nlm.nih.gov/pubmed/32120462
http://dx.doi.org/10.4014/jmb.2001.01049
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