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Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake
Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern A...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Microbiology and Biotechnology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728333/ https://www.ncbi.nlm.nih.gov/pubmed/32120462 http://dx.doi.org/10.4014/jmb.2001.01049 |
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author | Lee, Nan-Yeong Choi, Doo-Ho Kim, Mi-Gyeong Jeong, Min-Ji Kwon, Hae-Jun Kim, Dong-Hyun Kim, Young-Guk Luccio, Eric di Arioka, Manabu Yoon, Hyeok-Jun Kim, Jong-Guk |
author_facet | Lee, Nan-Yeong Choi, Doo-Ho Kim, Mi-Gyeong Jeong, Min-Ji Kwon, Hae-Jun Kim, Dong-Hyun Kim, Young-Guk Luccio, Eric di Arioka, Manabu Yoon, Hyeok-Jun Kim, Jong-Guk |
author_sort | Lee, Nan-Yeong |
collection | PubMed |
description | Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward. |
format | Online Article Text |
id | pubmed-9728333 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society for Microbiology and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97283332022-12-13 Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake Lee, Nan-Yeong Choi, Doo-Ho Kim, Mi-Gyeong Jeong, Min-Ji Kwon, Hae-Jun Kim, Dong-Hyun Kim, Young-Guk Luccio, Eric di Arioka, Manabu Yoon, Hyeok-Jun Kim, Jong-Guk J Microbiol Biotechnol Research article Tricholoma matsutake is an ectomycorrhizal fungus, related with the host of Pinus densiflora. Most of studies on T. matsutake have focused on mycelial growth, genes and genomics, phylogenetics, symbiosis, and immune activity of this strain. T. matsutake is known for its unique fragrance in Eastern Asia. The most major component of its scent is (R)-(-)-1-octen-3-ol and is biosynthesized from the substrate linoleic acid by the sequential reaction of lipoxygenase and peroxide lyase. Here, we report for the first time the biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake using the yeast Saccharomyces cerevisiae as a host. In this study, cDNA genes correlated with these reactions were cloned from T. matsutake, and expression studies of theses genes were carried out in the yeast Saccharomyces cerevisiae. The product of these genes expression study was carried out with Western blotting. The biosynthesis of (R)-(-)- 1-octen-3-ol of T. matsutake in recombinant Saccharomyces cerevisiae was subsequently identified with GC-MS chromatography analysis. The biosynthesis of (R)-(-)-1-octen-3-ol with S. cerevisiae represents a significant step forward. Korean Society for Microbiology and Biotechnology 2020-02-28 2020-02-17 /pmc/articles/PMC9728333/ /pubmed/32120462 http://dx.doi.org/10.4014/jmb.2001.01049 Text en Copyright©2020 by The Korean Society for Microbiology and Biotechnology https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research article Lee, Nan-Yeong Choi, Doo-Ho Kim, Mi-Gyeong Jeong, Min-Ji Kwon, Hae-Jun Kim, Dong-Hyun Kim, Young-Guk Luccio, Eric di Arioka, Manabu Yoon, Hyeok-Jun Kim, Jong-Guk Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake |
title | Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake |
title_full | Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake |
title_fullStr | Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake |
title_full_unstemmed | Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake |
title_short | Biosynthesis of (R)-(-)-1-Octen-3-ol in Recombinant Saccharomyces cerevisiae with Lipoxygenase-1 and Hydroperoxide Lyase Genes from Tricholoma matsutake |
title_sort | biosynthesis of (r)-(-)-1-octen-3-ol in recombinant saccharomyces cerevisiae with lipoxygenase-1 and hydroperoxide lyase genes from tricholoma matsutake |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728333/ https://www.ncbi.nlm.nih.gov/pubmed/32120462 http://dx.doi.org/10.4014/jmb.2001.01049 |
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