Cargando…

Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus

Platycodon grandiflorum root (Platycodi radix) saponins, platycosides, have been used as health supplements and food items for the treatment of respiratory disorders and pulmonary diseases. Deglycosylated saponins have been known to exert stronger biological effects than their glycosylated forms. In...

Descripción completa

Detalles Bibliográficos
Autores principales: Ju, Jung-Hun, Kang, Su-Hwan, Kim, Tae-Hun, Shin, Kyung-Chul, Oh, Deok-Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Microbiology and Biotechnology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728355/
https://www.ncbi.nlm.nih.gov/pubmed/32238760
http://dx.doi.org/10.4014/jmb.2001.01041
_version_ 1784845232564600832
author Ju, Jung-Hun
Kang, Su-Hwan
Kim, Tae-Hun
Shin, Kyung-Chul
Oh, Deok-Kun
author_facet Ju, Jung-Hun
Kang, Su-Hwan
Kim, Tae-Hun
Shin, Kyung-Chul
Oh, Deok-Kun
author_sort Ju, Jung-Hun
collection PubMed
description Platycodon grandiflorum root (Platycodi radix) saponins, platycosides, have been used as health supplements and food items for the treatment of respiratory disorders and pulmonary diseases. Deglycosylated saponins have been known to exert stronger biological effects than their glycosylated forms. In the present study, glycosylated platycosides in Platycodi radix extract were biotransformed into deglycosylated 3-O-β-D-glucopyranosyl platycosides, including 3-O-β-Dglucopyranosyl platycodigenin, 3-O-β-D-glucopyranosyl polygalacic acid, and 3-O-β-Dglucopyranosyl platyconic acid, by pectinase from Aspergillus aculeatus. This is the first report on the quantitative enzymatic production of 3-O-β-D-glucopyranosyl platycosides. The chemical structures of 3-O-β-D-glucopyranosyl platycosides were identified with LC/MS. Moreover, the biotransformation pathways of the three types of platycosides in Platycodi radix into 3-O-β-Dglucopyranosyl platycosides were established.
format Online
Article
Text
id pubmed-9728355
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Korean Society for Microbiology and Biotechnology
record_format MEDLINE/PubMed
spelling pubmed-97283552022-12-13 Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus Ju, Jung-Hun Kang, Su-Hwan Kim, Tae-Hun Shin, Kyung-Chul Oh, Deok-Kun J Microbiol Biotechnol Research article Platycodon grandiflorum root (Platycodi radix) saponins, platycosides, have been used as health supplements and food items for the treatment of respiratory disorders and pulmonary diseases. Deglycosylated saponins have been known to exert stronger biological effects than their glycosylated forms. In the present study, glycosylated platycosides in Platycodi radix extract were biotransformed into deglycosylated 3-O-β-D-glucopyranosyl platycosides, including 3-O-β-Dglucopyranosyl platycodigenin, 3-O-β-D-glucopyranosyl polygalacic acid, and 3-O-β-Dglucopyranosyl platyconic acid, by pectinase from Aspergillus aculeatus. This is the first report on the quantitative enzymatic production of 3-O-β-D-glucopyranosyl platycosides. The chemical structures of 3-O-β-D-glucopyranosyl platycosides were identified with LC/MS. Moreover, the biotransformation pathways of the three types of platycosides in Platycodi radix into 3-O-β-Dglucopyranosyl platycosides were established. Korean Society for Microbiology and Biotechnology 2020-06-28 2020-03-13 /pmc/articles/PMC9728355/ /pubmed/32238760 http://dx.doi.org/10.4014/jmb.2001.01041 Text en Copyright©2020 by The Korean Society for Microbiology and Biotechnology https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research article
Ju, Jung-Hun
Kang, Su-Hwan
Kim, Tae-Hun
Shin, Kyung-Chul
Oh, Deok-Kun
Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus
title Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus
title_full Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus
title_fullStr Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus
title_full_unstemmed Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus
title_short Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus
title_sort biotransformation of glycosylated saponins in balloon flower root extract into 3-o-β-d-glucopyranosyl platycosides by deglycosylation of pectinase from aspergillus aculeatus
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728355/
https://www.ncbi.nlm.nih.gov/pubmed/32238760
http://dx.doi.org/10.4014/jmb.2001.01041
work_keys_str_mv AT jujunghun biotransformationofglycosylatedsaponinsinballoonflowerrootextractinto3obdglucopyranosylplatycosidesbydeglycosylationofpectinasefromaspergillusaculeatus
AT kangsuhwan biotransformationofglycosylatedsaponinsinballoonflowerrootextractinto3obdglucopyranosylplatycosidesbydeglycosylationofpectinasefromaspergillusaculeatus
AT kimtaehun biotransformationofglycosylatedsaponinsinballoonflowerrootextractinto3obdglucopyranosylplatycosidesbydeglycosylationofpectinasefromaspergillusaculeatus
AT shinkyungchul biotransformationofglycosylatedsaponinsinballoonflowerrootextractinto3obdglucopyranosylplatycosidesbydeglycosylationofpectinasefromaspergillusaculeatus
AT ohdeokkun biotransformationofglycosylatedsaponinsinballoonflowerrootextractinto3obdglucopyranosylplatycosidesbydeglycosylationofpectinasefromaspergillusaculeatus