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Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice
Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide. Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer. One of the most representative bacteria involved in CRC is enter...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Korean Society for Microbiology and Biotechnology
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728371/ https://www.ncbi.nlm.nih.gov/pubmed/32958727 http://dx.doi.org/10.4014/jmb.2006.06034 |
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author | Cho, Hye-Won Rhee, Ki-Jong Eom, Yong-Bin |
author_facet | Cho, Hye-Won Rhee, Ki-Jong Eom, Yong-Bin |
author_sort | Cho, Hye-Won |
collection | PubMed |
description | Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide. Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer. One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes. We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition. Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg(-1) (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg(-1))-treated group. We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison. Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different. There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone. In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group. However, after given zerumbone, the taxonomic relative abundance was increased. These findings suggest that zerumbone not only influenced the microbial diversity and richness, but also could be helpful for enhancing the balance of gut microbial composition. In this work, we demonstrate that zerumbone could restore the composition of intestinal microbiota. |
format | Online Article Text |
id | pubmed-9728371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Korean Society for Microbiology and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-97283712022-12-13 Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice Cho, Hye-Won Rhee, Ki-Jong Eom, Yong-Bin J Microbiol Biotechnol Research article Colorectal cancer (CRC) is the leading cause of common malignant neoplasm worldwide. Many studies have analyzed compositions of gut microbiota associated with various diseases such as inflammatory bowel diseases (IBD) and colon cancer. One of the most representative bacteria involved in CRC is enterotoxigenic Bacteroides fragilis (ETBF), a species belonging to phylum Bacteroidetes. We used ETBF colonized mice with azoxymethane (AOM)/dextran sulphate sodium (DSS) and zerumbone, a compound with anti-bacterial effect, to determine whether zerumbone could restore intestinal microbiota composition. Four experimental groups of mice were used: sham, ETBF colonized AOM/DSS group, ETBF colonized AOM/DSS group zerumbone 60 mg kg(-1) (ETBF/AOM/ DSS + Z (60)), and only zerumbone (60 mg kg(-1))-treated group. We performed reversible dye terminators-based analysis of 16S rRNA gene region V3-V4 for group comparison. Microbiota compositions of ETBF/AOM/DSS + Z (60) group and ETBF colonized AOM/DSS group not given zerumbone were significantly different. There were more Bacteroides in ETBF/AOM/DSS + Z (60) group than those in ETBF colonized AOM/DSS group, suggesting that B. fragilis could be a normal flora activated by zerumbone. In addition, based on linear discriminant analysis of effect size (LEfSe) analysis, microbial diversity decreased significantly in the ETBF colonized AOM/DSS group. However, after given zerumbone, the taxonomic relative abundance was increased. These findings suggest that zerumbone not only influenced the microbial diversity and richness, but also could be helpful for enhancing the balance of gut microbial composition. In this work, we demonstrate that zerumbone could restore the composition of intestinal microbiota. Korean Society for Microbiology and Biotechnology 2020-11-28 2020-09-14 /pmc/articles/PMC9728371/ /pubmed/32958727 http://dx.doi.org/10.4014/jmb.2006.06034 Text en Copyright©2020 by The Korean Society for Microbiology and Biotechnology https://creativecommons.org/licenses/by/4.0/This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research article Cho, Hye-Won Rhee, Ki-Jong Eom, Yong-Bin Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice |
title | Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice |
title_full | Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice |
title_fullStr | Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice |
title_full_unstemmed | Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice |
title_short | Zerumbone Restores Gut Microbiota Composition in ETBF Colonized AOM/DSS Mice |
title_sort | zerumbone restores gut microbiota composition in etbf colonized aom/dss mice |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728371/ https://www.ncbi.nlm.nih.gov/pubmed/32958727 http://dx.doi.org/10.4014/jmb.2006.06034 |
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