Cargando…

Identification and characterization of Colletotrichum fioriniae and C. fructicola that cause anthracnose in pecan

Pecan (Carya illinoinensis Wang. K. Koch) is a deciduous tree of the Juglandaceae family with important economic value worldwide. Anthracnose of the pecan leaves and shuck is a devastating disease faced by pecan-growing areas in China. However, the causal species occurring on pecan remain largely un...

Descripción completa

Detalles Bibliográficos
Autores principales: Chang, Jun, Zhai, Fengyan, Zhang, Yabo, Wang, Di, Shu, Jinping, Yao, Xiaohua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728526/
https://www.ncbi.nlm.nih.gov/pubmed/36507420
http://dx.doi.org/10.3389/fpls.2022.1043750
_version_ 1784845276295462912
author Chang, Jun
Zhai, Fengyan
Zhang, Yabo
Wang, Di
Shu, Jinping
Yao, Xiaohua
author_facet Chang, Jun
Zhai, Fengyan
Zhang, Yabo
Wang, Di
Shu, Jinping
Yao, Xiaohua
author_sort Chang, Jun
collection PubMed
description Pecan (Carya illinoinensis Wang. K. Koch) is a deciduous tree of the Juglandaceae family with important economic value worldwide. Anthracnose of the pecan leaves and shuck is a devastating disease faced by pecan-growing areas in China. However, the causal species occurring on pecan remain largely unidentified. we collected samples of diseased pecan from the provinces of China, Leaves and fruits affected by anthracnose were sampled and subjected to fungus isolation, The morphological characters of all strains were observed and compared; Multi-locus phylogenetic analyses [Internally transcribed spacer (ITS), Actin (ACT), Calmodulin (CAL), Chitin synthase (CHS1), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and b-tubulin (TUB2)] were performed on selected representative strains; examine their pathogenicity on leaves of pecan.The results showed that: (1) resulting in a total of 11 Colletotrichum isolates, Two Colletotrichum species were identifified to be C. fioriniae and C. fructicola; (2) Pathogenicity tests revealed that both species caused black spots on pecan leaves and fruit, The virulence of the different isolates varied substantially, with C. fioriniae PCJD179 being the most virulent; (3) The susceptibility levels of pecan tree varieties, ‘Mahan’ and ‘Kanza’, were determined, No significant differences were observed in the lesion sizes produced by the various isolates in ‘Kanza’, while there were signifificant differences in ‘Mahan’. This study is thefifirst to determine that C. fructicola and C. fioriniaecan cause anthracnose in pecan in China. It improves the understanding of the species that cause anthracnose in pecan and provides useful information for the effective control of this disease in China.
format Online
Article
Text
id pubmed-9728526
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97285262022-12-08 Identification and characterization of Colletotrichum fioriniae and C. fructicola that cause anthracnose in pecan Chang, Jun Zhai, Fengyan Zhang, Yabo Wang, Di Shu, Jinping Yao, Xiaohua Front Plant Sci Plant Science Pecan (Carya illinoinensis Wang. K. Koch) is a deciduous tree of the Juglandaceae family with important economic value worldwide. Anthracnose of the pecan leaves and shuck is a devastating disease faced by pecan-growing areas in China. However, the causal species occurring on pecan remain largely unidentified. we collected samples of diseased pecan from the provinces of China, Leaves and fruits affected by anthracnose were sampled and subjected to fungus isolation, The morphological characters of all strains were observed and compared; Multi-locus phylogenetic analyses [Internally transcribed spacer (ITS), Actin (ACT), Calmodulin (CAL), Chitin synthase (CHS1), Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and b-tubulin (TUB2)] were performed on selected representative strains; examine their pathogenicity on leaves of pecan.The results showed that: (1) resulting in a total of 11 Colletotrichum isolates, Two Colletotrichum species were identifified to be C. fioriniae and C. fructicola; (2) Pathogenicity tests revealed that both species caused black spots on pecan leaves and fruit, The virulence of the different isolates varied substantially, with C. fioriniae PCJD179 being the most virulent; (3) The susceptibility levels of pecan tree varieties, ‘Mahan’ and ‘Kanza’, were determined, No significant differences were observed in the lesion sizes produced by the various isolates in ‘Kanza’, while there were signifificant differences in ‘Mahan’. This study is thefifirst to determine that C. fructicola and C. fioriniaecan cause anthracnose in pecan in China. It improves the understanding of the species that cause anthracnose in pecan and provides useful information for the effective control of this disease in China. Frontiers Media S.A. 2022-11-23 /pmc/articles/PMC9728526/ /pubmed/36507420 http://dx.doi.org/10.3389/fpls.2022.1043750 Text en Copyright © 2022 Chang, Zhai, Zhang, Wang, Shu and Yao https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Chang, Jun
Zhai, Fengyan
Zhang, Yabo
Wang, Di
Shu, Jinping
Yao, Xiaohua
Identification and characterization of Colletotrichum fioriniae and C. fructicola that cause anthracnose in pecan
title Identification and characterization of Colletotrichum fioriniae and C. fructicola that cause anthracnose in pecan
title_full Identification and characterization of Colletotrichum fioriniae and C. fructicola that cause anthracnose in pecan
title_fullStr Identification and characterization of Colletotrichum fioriniae and C. fructicola that cause anthracnose in pecan
title_full_unstemmed Identification and characterization of Colletotrichum fioriniae and C. fructicola that cause anthracnose in pecan
title_short Identification and characterization of Colletotrichum fioriniae and C. fructicola that cause anthracnose in pecan
title_sort identification and characterization of colletotrichum fioriniae and c. fructicola that cause anthracnose in pecan
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9728526/
https://www.ncbi.nlm.nih.gov/pubmed/36507420
http://dx.doi.org/10.3389/fpls.2022.1043750
work_keys_str_mv AT changjun identificationandcharacterizationofcolletotrichumfioriniaeandcfructicolathatcauseanthracnoseinpecan
AT zhaifengyan identificationandcharacterizationofcolletotrichumfioriniaeandcfructicolathatcauseanthracnoseinpecan
AT zhangyabo identificationandcharacterizationofcolletotrichumfioriniaeandcfructicolathatcauseanthracnoseinpecan
AT wangdi identificationandcharacterizationofcolletotrichumfioriniaeandcfructicolathatcauseanthracnoseinpecan
AT shujinping identificationandcharacterizationofcolletotrichumfioriniaeandcfructicolathatcauseanthracnoseinpecan
AT yaoxiaohua identificationandcharacterizationofcolletotrichumfioriniaeandcfructicolathatcauseanthracnoseinpecan