Structural basis for the non-self RNA-activated protease activity of the type III-E CRISPR nuclease-protease Craspase

The RNA-targeting type III-E CRISPR-gRAMP effector interacts with a caspase-like protease TPR-CHAT to form the CRISPR-guided caspase complex (Craspase), but their functional mechanism is unknown. Here, we report cryo-EM structures of the type III-E gRAMP(crRNA) and gRAMP(crRNA)-TPR-CHAT complexes, b...

Descripción completa

Detalles Bibliográficos
Autores principales: Cui, Ning, Zhang, Jun-Tao, Li, Zhuolin, Liu, Xiao-Yu, Wang, Chongyuan, Huang, Hongda, Jia, Ning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729208/
https://www.ncbi.nlm.nih.gov/pubmed/36477448
http://dx.doi.org/10.1038/s41467-022-35275-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729208/
https://www.ncbi.nlm.nih.gov/pubmed/36477448
http://dx.doi.org/10.1038/s41467-022-35275-5

Ejemplares similares