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Structural basis for the non-self RNA-activated protease activity of the type III-E CRISPR nuclease-protease Craspase
The RNA-targeting type III-E CRISPR-gRAMP effector interacts with a caspase-like protease TPR-CHAT to form the CRISPR-guided caspase complex (Craspase), but their functional mechanism is unknown. Here, we report cryo-EM structures of the type III-E gRAMP(crRNA) and gRAMP(crRNA)-TPR-CHAT complexes, b...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729208/ https://www.ncbi.nlm.nih.gov/pubmed/36477448 http://dx.doi.org/10.1038/s41467-022-35275-5 |