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Age-associated B cells are long-lasting effectors that impede latent γHV68 reactivation

Age-associated B cells (ABCs; CD19(+)CD11c(+)T-bet(+)) are a unique population that are increased in an array of viral infections, though their role during latent infection is largely unexplored. Here, we use murine gammaherpesvirus 68 (γHV68) to demonstrate that ABCs remain elevated long-term durin...

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Autores principales: Mouat, Isobel C., Shanina, Iryna, Horwitz, Marc S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729602/
https://www.ncbi.nlm.nih.gov/pubmed/36477199
http://dx.doi.org/10.1038/s41598-022-25543-1
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author Mouat, Isobel C.
Shanina, Iryna
Horwitz, Marc S.
author_facet Mouat, Isobel C.
Shanina, Iryna
Horwitz, Marc S.
author_sort Mouat, Isobel C.
collection PubMed
description Age-associated B cells (ABCs; CD19(+)CD11c(+)T-bet(+)) are a unique population that are increased in an array of viral infections, though their role during latent infection is largely unexplored. Here, we use murine gammaherpesvirus 68 (γHV68) to demonstrate that ABCs remain elevated long-term during latent infection and express IFNγ and TNF. Using a recombinant γHV68 that is cleared following acute infection, we show that ABCs persist in the absence of latent virus, though their expression of IFNγ and TNF is decreased. With a fluorescent reporter gene-expressing γHV68 we demonstrate that ABCs are infected with γHV68 at similar rates to other previously activated B cells. We find that mice without ABCs display defects in anti-viral IgG2a/c antibodies and are more susceptible to reactivation of γHV68 following virus challenges that typically do not break latency. Together, these results indicate that ABCs are a persistent effector subset during latent viral infection that impedes γHV68 reactivation.
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spelling pubmed-97296022022-12-09 Age-associated B cells are long-lasting effectors that impede latent γHV68 reactivation Mouat, Isobel C. Shanina, Iryna Horwitz, Marc S. Sci Rep Article Age-associated B cells (ABCs; CD19(+)CD11c(+)T-bet(+)) are a unique population that are increased in an array of viral infections, though their role during latent infection is largely unexplored. Here, we use murine gammaherpesvirus 68 (γHV68) to demonstrate that ABCs remain elevated long-term during latent infection and express IFNγ and TNF. Using a recombinant γHV68 that is cleared following acute infection, we show that ABCs persist in the absence of latent virus, though their expression of IFNγ and TNF is decreased. With a fluorescent reporter gene-expressing γHV68 we demonstrate that ABCs are infected with γHV68 at similar rates to other previously activated B cells. We find that mice without ABCs display defects in anti-viral IgG2a/c antibodies and are more susceptible to reactivation of γHV68 following virus challenges that typically do not break latency. Together, these results indicate that ABCs are a persistent effector subset during latent viral infection that impedes γHV68 reactivation. Nature Publishing Group UK 2022-12-07 /pmc/articles/PMC9729602/ /pubmed/36477199 http://dx.doi.org/10.1038/s41598-022-25543-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mouat, Isobel C.
Shanina, Iryna
Horwitz, Marc S.
Age-associated B cells are long-lasting effectors that impede latent γHV68 reactivation
title Age-associated B cells are long-lasting effectors that impede latent γHV68 reactivation
title_full Age-associated B cells are long-lasting effectors that impede latent γHV68 reactivation
title_fullStr Age-associated B cells are long-lasting effectors that impede latent γHV68 reactivation
title_full_unstemmed Age-associated B cells are long-lasting effectors that impede latent γHV68 reactivation
title_short Age-associated B cells are long-lasting effectors that impede latent γHV68 reactivation
title_sort age-associated b cells are long-lasting effectors that impede latent γhv68 reactivation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9729602/
https://www.ncbi.nlm.nih.gov/pubmed/36477199
http://dx.doi.org/10.1038/s41598-022-25543-1
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