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An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting
The ability to regulate, maintain and reproduce fluorogenic properties is a fundamental prerequisite of modern molecular diagnostics, nanotechnology and bioimaging. The sequence-dependence of the fluorescence properties in fluorophores commonly used in nucleic acid labelling is here being exploited...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730302/ https://www.ncbi.nlm.nih.gov/pubmed/36416340 http://dx.doi.org/10.1039/d2nr05269e |
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author | Kekić, Tadija Lietard, Jory |
author_facet | Kekić, Tadija Lietard, Jory |
author_sort | Kekić, Tadija |
collection | PubMed |
description | The ability to regulate, maintain and reproduce fluorogenic properties is a fundamental prerequisite of modern molecular diagnostics, nanotechnology and bioimaging. The sequence-dependence of the fluorescence properties in fluorophores commonly used in nucleic acid labelling is here being exploited to assemble a color scale in 256 shades of green Cy3 fluorescence. Using photolithography, we synthesize microarrays of labeled nucleic acids that can accurately reproduce 8-bit monochrome graphics by mapping color to fluorescence intensity and sequence. This DNA-based painting approach paves the way for a full RGB scale array fabrication process. |
format | Online Article Text |
id | pubmed-9730302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-97303022022-12-19 An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting Kekić, Tadija Lietard, Jory Nanoscale Chemistry The ability to regulate, maintain and reproduce fluorogenic properties is a fundamental prerequisite of modern molecular diagnostics, nanotechnology and bioimaging. The sequence-dependence of the fluorescence properties in fluorophores commonly used in nucleic acid labelling is here being exploited to assemble a color scale in 256 shades of green Cy3 fluorescence. Using photolithography, we synthesize microarrays of labeled nucleic acids that can accurately reproduce 8-bit monochrome graphics by mapping color to fluorescence intensity and sequence. This DNA-based painting approach paves the way for a full RGB scale array fabrication process. The Royal Society of Chemistry 2022-11-18 /pmc/articles/PMC9730302/ /pubmed/36416340 http://dx.doi.org/10.1039/d2nr05269e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Kekić, Tadija Lietard, Jory An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting |
title | An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting |
title_full | An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting |
title_fullStr | An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting |
title_full_unstemmed | An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting |
title_short | An 8-bit monochrome palette of fluorescent nucleic acid sequences for DNA-based painting |
title_sort | 8-bit monochrome palette of fluorescent nucleic acid sequences for dna-based painting |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730302/ https://www.ncbi.nlm.nih.gov/pubmed/36416340 http://dx.doi.org/10.1039/d2nr05269e |
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