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Transcription factor c-Jun modulates GLUT1 in glycolysis and breast cancer metastasis
As the main isoforms of membranous glucose transporters (GLUT), GLUT1 involves tumorigenesis, metastasis and prognosis in a variety of cancers. However, its role in breast cancer metastasis remains to be elucidated. Here we examined its transcriptional and survival data in patients with breast cance...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730598/ https://www.ncbi.nlm.nih.gov/pubmed/36476606 http://dx.doi.org/10.1186/s12885-022-10393-x |
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author | Zhu, Ping Liu, Guoping Wang, Xue Lu, Jingjing Zhou, Yue Chen, Shuyi Gao, Yabiao Wang, Chaofu Yu, Jerry Sun, Yangbai Zhou, Ping |
author_facet | Zhu, Ping Liu, Guoping Wang, Xue Lu, Jingjing Zhou, Yue Chen, Shuyi Gao, Yabiao Wang, Chaofu Yu, Jerry Sun, Yangbai Zhou, Ping |
author_sort | Zhu, Ping |
collection | PubMed |
description | As the main isoforms of membranous glucose transporters (GLUT), GLUT1 involves tumorigenesis, metastasis and prognosis in a variety of cancers. However, its role in breast cancer metastasis remains to be elucidated. Here we examined its transcriptional and survival data in patients with breast cancer from several independent databases including the Oncomine, Gene Expression Profiling Interactive Analysis, Gene Expression across Normal and Tumor tissue, UALCAN, cBioPortal, Kaplan-Meier Plotter and PROGgeneV2. We found that its mRNA expression was significantly high in cancer tissues, which was associated with metastasis and poor survival. Transcription factor c-Jun might bind to GLUT1 promoter to downregulate its gene expression or mRNA stability, therefore to suppress glycolysis and metastasis. By qRT-PCR, we verified that GLUT1 was significantly increased in 38 paired human breast cancer samples while JUN was decreased. Furthermore, the protein level of GLUT1 was higher in tumor than in normal tissues by IHC assay. To explore underlying pathways, we further performed GO and KEGG analysis of genes related to GLUT1 and JUN and found that GLUT1 was increased by transcription factor c-Jun in breast cancer tissues to influence glycolysis and breast cancer metastasis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-022-10393-x. |
format | Online Article Text |
id | pubmed-9730598 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-97305982022-12-09 Transcription factor c-Jun modulates GLUT1 in glycolysis and breast cancer metastasis Zhu, Ping Liu, Guoping Wang, Xue Lu, Jingjing Zhou, Yue Chen, Shuyi Gao, Yabiao Wang, Chaofu Yu, Jerry Sun, Yangbai Zhou, Ping BMC Cancer Research As the main isoforms of membranous glucose transporters (GLUT), GLUT1 involves tumorigenesis, metastasis and prognosis in a variety of cancers. However, its role in breast cancer metastasis remains to be elucidated. Here we examined its transcriptional and survival data in patients with breast cancer from several independent databases including the Oncomine, Gene Expression Profiling Interactive Analysis, Gene Expression across Normal and Tumor tissue, UALCAN, cBioPortal, Kaplan-Meier Plotter and PROGgeneV2. We found that its mRNA expression was significantly high in cancer tissues, which was associated with metastasis and poor survival. Transcription factor c-Jun might bind to GLUT1 promoter to downregulate its gene expression or mRNA stability, therefore to suppress glycolysis and metastasis. By qRT-PCR, we verified that GLUT1 was significantly increased in 38 paired human breast cancer samples while JUN was decreased. Furthermore, the protein level of GLUT1 was higher in tumor than in normal tissues by IHC assay. To explore underlying pathways, we further performed GO and KEGG analysis of genes related to GLUT1 and JUN and found that GLUT1 was increased by transcription factor c-Jun in breast cancer tissues to influence glycolysis and breast cancer metastasis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-022-10393-x. BioMed Central 2022-12-07 /pmc/articles/PMC9730598/ /pubmed/36476606 http://dx.doi.org/10.1186/s12885-022-10393-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Zhu, Ping Liu, Guoping Wang, Xue Lu, Jingjing Zhou, Yue Chen, Shuyi Gao, Yabiao Wang, Chaofu Yu, Jerry Sun, Yangbai Zhou, Ping Transcription factor c-Jun modulates GLUT1 in glycolysis and breast cancer metastasis |
title | Transcription factor c-Jun modulates GLUT1 in glycolysis and breast cancer metastasis |
title_full | Transcription factor c-Jun modulates GLUT1 in glycolysis and breast cancer metastasis |
title_fullStr | Transcription factor c-Jun modulates GLUT1 in glycolysis and breast cancer metastasis |
title_full_unstemmed | Transcription factor c-Jun modulates GLUT1 in glycolysis and breast cancer metastasis |
title_short | Transcription factor c-Jun modulates GLUT1 in glycolysis and breast cancer metastasis |
title_sort | transcription factor c-jun modulates glut1 in glycolysis and breast cancer metastasis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730598/ https://www.ncbi.nlm.nih.gov/pubmed/36476606 http://dx.doi.org/10.1186/s12885-022-10393-x |
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