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The double round-robin population unravels the genetic architecture of grain size in barley

Grain number, size and weight primarily determine the yield of barley. Although the genes regulating grain number are well studied in barley, the genetic loci and the causal gene for sink capacity are poorly understood. Therefore, the primary objective of our work was to dissect the genetic architec...

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Autores principales: Shrestha, Asis, Cosenza, Francesco, van Inghelandt, Delphine, Wu, Po-Ya, Li, Jinquan, Casale, Federico A, Weisweiler, Marius, Stich, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730814/
https://www.ncbi.nlm.nih.gov/pubmed/36094852
http://dx.doi.org/10.1093/jxb/erac369
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author Shrestha, Asis
Cosenza, Francesco
van Inghelandt, Delphine
Wu, Po-Ya
Li, Jinquan
Casale, Federico A
Weisweiler, Marius
Stich, Benjamin
author_facet Shrestha, Asis
Cosenza, Francesco
van Inghelandt, Delphine
Wu, Po-Ya
Li, Jinquan
Casale, Federico A
Weisweiler, Marius
Stich, Benjamin
author_sort Shrestha, Asis
collection PubMed
description Grain number, size and weight primarily determine the yield of barley. Although the genes regulating grain number are well studied in barley, the genetic loci and the causal gene for sink capacity are poorly understood. Therefore, the primary objective of our work was to dissect the genetic architecture of grain size and weight in barley. We used a multi-parent population developed from a genetic cross between 23 diverse barley inbreds in a double round-robin design. Seed size-related parameters such as grain length, grain width, grain area and thousand-grain weight were evaluated in the HvDRR population comprising 45 recombinant inbred line sub-populations. We found significant genotypic variation for all seed size characteristics, and observed 84% or higher heritability across four environments. The quantitative trait locus (QTL) detection results indicate that the genetic architecture of grain size is more complex than previously reported. In addition, both cultivars and landraces contributed positive alleles at grain size QTLs. Candidate genes identified using genome-wide variant calling data for all parental inbred lines indicated overlapping and potential novel regulators of grain size in cereals. Furthermore, our results indicated that sink capacity was the primary determinant of grain weight in barley.
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spelling pubmed-97308142022-12-13 The double round-robin population unravels the genetic architecture of grain size in barley Shrestha, Asis Cosenza, Francesco van Inghelandt, Delphine Wu, Po-Ya Li, Jinquan Casale, Federico A Weisweiler, Marius Stich, Benjamin J Exp Bot Research Papers Grain number, size and weight primarily determine the yield of barley. Although the genes regulating grain number are well studied in barley, the genetic loci and the causal gene for sink capacity are poorly understood. Therefore, the primary objective of our work was to dissect the genetic architecture of grain size and weight in barley. We used a multi-parent population developed from a genetic cross between 23 diverse barley inbreds in a double round-robin design. Seed size-related parameters such as grain length, grain width, grain area and thousand-grain weight were evaluated in the HvDRR population comprising 45 recombinant inbred line sub-populations. We found significant genotypic variation for all seed size characteristics, and observed 84% or higher heritability across four environments. The quantitative trait locus (QTL) detection results indicate that the genetic architecture of grain size is more complex than previously reported. In addition, both cultivars and landraces contributed positive alleles at grain size QTLs. Candidate genes identified using genome-wide variant calling data for all parental inbred lines indicated overlapping and potential novel regulators of grain size in cereals. Furthermore, our results indicated that sink capacity was the primary determinant of grain weight in barley. Oxford University Press 2022-09-12 /pmc/articles/PMC9730814/ /pubmed/36094852 http://dx.doi.org/10.1093/jxb/erac369 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Shrestha, Asis
Cosenza, Francesco
van Inghelandt, Delphine
Wu, Po-Ya
Li, Jinquan
Casale, Federico A
Weisweiler, Marius
Stich, Benjamin
The double round-robin population unravels the genetic architecture of grain size in barley
title The double round-robin population unravels the genetic architecture of grain size in barley
title_full The double round-robin population unravels the genetic architecture of grain size in barley
title_fullStr The double round-robin population unravels the genetic architecture of grain size in barley
title_full_unstemmed The double round-robin population unravels the genetic architecture of grain size in barley
title_short The double round-robin population unravels the genetic architecture of grain size in barley
title_sort double round-robin population unravels the genetic architecture of grain size in barley
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9730814/
https://www.ncbi.nlm.nih.gov/pubmed/36094852
http://dx.doi.org/10.1093/jxb/erac369
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